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Biology of Reproduction, Vol 21, 839-844, Copyright © 1979 by Society for the Study of Reproduction

Fertilization in vitro of Rat Ovarian Oocytes after Freezing and Thawing

M. KASAI 1, A. IRITANI 1, , and M. C. CHANG 2

1 Department of Animal Science, College of Agriculture, Kyoto University, Kyoto 606, Japan
2 Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545


Rat ovarian oocytes recovered from immature females 48 h after an injection of PMSG and 3, 6.5 and 8.5 h after an injection of hCG were frozen at -196°C. After thawing they were cultured for 14, 11, 7.5 and 5.5 h, respectively, and then inseminated with epididymal spermatozoa. When dimethylsulphoxide (DMSO) was added at 0°C, 46-54% of the oocytes recovered without injection of hCG were normal after thawing and culture; 33-34% of the normal oocytes were penetrated following insemination in the samples in which DMSO was diluted at room temperature or 37°C after thawing. However, when DMSO was diluted at room temperature, comparatively higher proportions of normal oocytes (63%) and of penetrated oocytes (47%) were observed in the samples to which DMSO was added at room temperature and 37°C, respectively. With increasing time of oocyte collection after injection of hCG, the proportions of normal oocytes cultured after thawing and of penetrated oocytes after insemination were progressively increased when DMSO was added and diluted at room temperature.

Note:
ACKNOWLEDGMENTS M. Kasai was supported by a grant (HD 03472) from NICHD when he started this study at the Worcester Foundation for Experimental Biology.

Submitted on March 30, 1979
Accepted on July 23, 1979




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 Hum Reprod UpdateHome page
D. A. Gook and D. H. Edgar
Human oocyte cryopreservation
Hum. Reprod. Update, November 1, 2007; 13(6): 591 - 605.
[Abstract] [Full Text] [PDF]


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Copyright © 1979 by the Society for the Study of Reproduction.