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Biology of Reproduction, Vol 21, 1175-1183, Copyright © 1979 by Society for the Study of Reproduction
on Cyclic AMP Accumulation in Rat Corpora Lutea of Various Ages
1 Department of Physiology,
University of Göteborg,
Göteborg, Sweden In vivo administration of prostaglandin F2 In vitro administration of PGF2 A potent phosphodiesterase inhibitor, IBMX, had no effect on the PGF2 The present results illustrate that the inhibitory effect of PGF2
(PGF2) to PMSG treated immature rats drastically
reduced the in vitro effect of LH on cAMP accumulation in 7-day-old corpora lutea (CL) but failed
to do so in 3-day-old CL. added simultaneously with LH, reduced the LH effect on
cAMP accumulation in 7- and 3-day-old CL but not in 1-day-old CL. A concentration of PGF2 as
low as 0.05 µM significantly inhibited the LH induced rise in cAMP accumulation in 7-day-old CL.
A 100 times higher concentration of PGF2 was needed to reduce the LH effect in 3-day-old CL,
while 1-day-old CL were completely resistant to the inhibitory effect of PGF2.-induced decrease of
the LH effect. Furthermore, no change in the phosphodiesterase enzyme activity was observed in
the CL after PGF2 treatment. PGF2, when tested in combination with PGE2, did not inhibit the
increase in cAMP produced by PGE2. on the LH-induced rise in
cAMP accumulation is highly dependent upon the age of the CL and that this effect of PGF2 is
not exerted through changes in the phosphodiesterase enzyme activity. These results give further
support to the possibility that changes in the cAMP response are one of the earliest steps in PGF2 -induced luteolysis.
Note:
ACKNOWLEDGMENTS
We express our thanks to Miss Barbro Henskog and
Stina Öberg for their expert technical assistance, Mrs.
Gun Derevall and Mrs. Britt-Marie Udder for typing
the manuscript and NIH, USA for the generous supply
of LH. Michal Lahav and Sergio Lainprecht gratefully
acknowledge the travel grant from EMBO. This work
was supported by grants from the Swedish Medical
Research Council (14X-00027), the Ford Foundation
(76-0082), AB Leo Research Foundation, the Magnus Bergvall's Foundation and the Medical Faculty,
University of Göteborg, Sweden.
This article has been cited by other articles:
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  A. Zetser, T. Kisliouk, E. Ivakin, and M. Lahav Dependence on Prolactin of the Luteolytic Effect of Prostaglandin F2{alpha} in Rat Luteal Cell Cultures Biol Reprod, October 1, 2001; 65(4): 1082 - 1091. [Abstract] [Full Text] [PDF]
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 A. B. Motta, A. Estevez, and M. F. de Gimeno The involvement of nitric oxide in corpus luteum regression in the rat: feedback mechanism between prostaglandin F2{alpha} and nitric oxide Mol. Hum. Reprod., November 1, 1999; 5(11): 1011 - 1016. [Abstract] [Full Text] [PDF]
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