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Biology of Reproduction, Vol 22, 281-288, Copyright © 1980 by Society for the Study of Reproduction

Immunocytochemical Localization of Cyclic AMP and Cyclic GMP in Rat Ovarian Tissue

WILLIAM G. DAIL 1, CAROLYN R. SANBORN 1, , and ALBERT RATNER 1

1 Departments of Anatomy and Physiology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131


The localization of cyclic 3',5'-adenosine monophosphate (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) in ovarian tissue from immature rats treated with pregnant mares serum gonadotropin (PMSG) or PMSG and human chorionic gonadotropin (hCG) was examined using fluorescent immunocytochemical procedures. Within individual cells the staining patterns for cAMP and cGMP are quite distinct. Cyclic AMP was localized in the cytoplasm of the various ovarian cell types. Among the granulosa cells, there was an apparent variability of staining intensity. An increased fluorescence was observed in granulosa cells bordering the antrum of large follicles. This distribution pattern suggests that this nucleotide may be involved in cellular events related to the maturation of the follicle. Cyclic GMP was predominantly located in the nucleus of luteal cells and granulosa cells of large follicles. The nuclear localization in these cells suggests a role for this nucleotide in nucleus directed events. An intense reaction for cGMP was also found in the cytoplasm of perifollicular smooth muscle cells, suggesting that cGMP may be involved in the contraction of these cells. This methodology appears useful for determining the cell type, in heterogeneous ovarian tissue, in which changes in cyclic nucleotides occur. The observations provide histological evidence which suggests diverse roles for the two nucleotides in ovarian function.

Submitted on August 7, 1979
Accepted on October 2, 1979




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