Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by OLSON, G. E.
Right arrow Articles by SAMMONS, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by OLSON, G. E.
Right arrow Articles by SAMMONS, D. W.
Agricola
Right arrow Articles by OLSON, G. E.
Right arrow Articles by SAMMONS, D. W.

Biology of Reproduction, Vol 22, 319-332, Copyright © 1980 by Society for the Study of Reproduction

Structural Chemistry of Outer Dense Fibers of Rat Sperm

GARY E. OLSON 1, and DAVID W. SAMMONS 2

1 Department of Anatomy, Vanderbilt University, Nashville, Tennessee 37232
2 Department of Medicine, Division of Human Genetics, The New York Hospital-Cornell Medical Center, New York, New York 10021


Rat sperm outer dense fibers (ODF) have been studied by several electron microscopic techniques to determine fine structural features of the ODF cortex and medulla. Cross sections of Triton X-100 demembranated flagella, fixed in the presence of ruthenium red, reveal a well defined ODF cortex which appears to be composed of a single lamina of 6-7 nm diameter globular particles, whereas the medulla appears electron dense with no obvious substructure. Surface replicas and positively or negatively stained whole mount preparations reveal a crossbanding pattern with a 40 nm major period repeat that extends obliquely over the outer dense fiber surface. These periodicities appear to be confined to the outer dense fiber cortex.

Sequential solubilization of spermatozoan organelles in sodium dodecyl sulfate or sodium lauroyl sacrosine results in the isolation of a flagellar subfraction containing only the ODF and attached connecting piece. Further solubilization of the complex in the ionic detergents results in an ODF subfraction which contains 4 polypeptide components. The 3 low molecular weight polypeptide bands have a high cysteine content (10-12%) while the high molecular weight component (87,000 daltons) contains sim3% cysteine. Brief trypsin or chymotrypsin digestion of demembranated flagella gives an ODF subfraction which by SDS polyacrylamide gel electrophoresis is shown to possess only the 3 low molecular weight polypeptide bands. These ODFs appear to possess an intact medulla but are lacking a cortex, thereby suggesting that the low molecular weight components are located in the medulla.

 Submitted on June 26, 1979
Accepted on October 31, 1979




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
K. Tokuhiro, M. Hirose, Y. Miyagawa, A. Tsujimura, S. Irie, A. Isotani, M. Okabe, Y. Toyama, C. Ito, K. Toshimori, et al.
Meichroacidin Containing the Membrane Occupation and Recognition Nexus Motif Is Essential for Spermatozoa Morphogenesis
J. Biol. Chem., July 4, 2008; 283(27): 19039 - 19048.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
F. F. Donkor, M. Monnich, E. Czirr, T. Hollemann, and S. Hoyer-Fender
Outer dense fibre protein 2 (ODF2) is a self-interacting centrosomal protein with affinity for microtubules
J. Cell Sci., September 15, 2004; 117(20): 4643 - 4651.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. L. Kierszenbaum, E. Rivkin, and L. L. Tres
Acroplaxome, an F-Actin-Keratin-containing Plate, Anchors the Acrosome to the Nucleus during Shaping of the Spermatid Head
Mol. Biol. Cell, November 1, 2003; 14(11): 4628 - 4640.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. Kitamura, Y. Miyagawa, N. Iguchi, H. Nishimura, H. Tanaka, and Y. Nishimune
Molecular cloning and characterization of the human orthologue of the oppo 1 gene encoding a sperm tail protein
Mol. Hum. Reprod., May 1, 2003; 9(5): 237 - 243.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. A. Zarsky, M. Cheng, and F. A. van der Hoorn
Novel RING Finger Protein OIP1 Binds to Conserved Amino Acid Repeats in Sperm Tail Protein ODF1
Biol Reprod, February 1, 2003; 68(2): 543 - 552.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
J. Xue, H. A. Tarnasky, D. E. Rancourt, and F. A. van der Hoorn
Targeted Disruption of the Testicular SPAG5/Deepest Protein Does Not Affect Spermatogenesis or Fertility
Mol. Cell. Biol., April 1, 2002; 22(7): 1993 - 1997.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Egydio de Carvalho, H. Tanaka, N. Iguchi, S. Ventela, H. Nojima, and Y. Nishimune
Molecular Cloning and Characterization of a Complementary DNA Encoding Sperm Tail Protein SHIPPO 1
Biol Reprod, March 1, 2002; 66(3): 785 - 795.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. J. Flickinger, J. Rao, L. Ann Bush, N. E. Sherman, R. J. Oko, F. C.L. Jayes, and J. C. Herr
Outer Dense Fiber Proteins Are Dominant Postobstruction Autoantigens in Adult Lewis Rats
Biol Reprod, May 1, 2001; 64(5): 1451 - 1459.
[Abstract] [Full Text]

Home page
 Cell Growth Differ.Home page
V. Besset, K. Rhee, and D. J. Wolgemuth
The Cellular Distribution and Kinase Activity of the Cdk Family Member Pctaire1 in the Adult Mouse Brain and Testis Suggest Functions in Differentiation
Cell Growth Differ., March 1, 1999; 10(3): 173 - 181.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
H. Brohmann, S. Pinnecke, and S. Hoyer-Fender
Identification and Characterization of New cDNAs Encoding Outer Dense Fiber Proteins of Rat Sperm
J. Biol. Chem., April 11, 1997; 272(15): 10327 - 10332.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Shao, H. A. Tarnasky, U. Schalles, R. Oko, and F. A. van der Hoorn
Interactional Cloning of the 84-kDa Major Outer Dense Fiber Protein Odf84. LEUCINE ZIPPERS MEDIATE ASSOCIATIONS OF Odf84 AND Odf27
J. Biol. Chem., March 7, 1997; 272(10): 6105 - 6113.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the Society for the Study of Reproduction.