Biology of Reproduction, Vol 22, 759-764, Copyright © 1980 by Society for the Study of Reproduction

Movement Characteristics of Hamster Spermatozoa Within the Oviduct

¹ Department of Obstetrics and Gynecology, School of Medicine, University of California, Davis, California 95616
² Department of Anatomy and Reproductive Biology, The John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii 96822

Movement characteristics of golden hamster spermatozoa within the oviduct at the time and site of fertilization were studied using high-speed cinemicrography. Preparations consisted of excised oviducts immediately submersed in mineral oil and placed in a deep slide-coverglass system at 37°C. All spermatozoa visualized were motile and were exhibiting flagellar movement similar to the "activated" motility previously identified by observations in vitro. The geometrical details of this movement were not constant with time, however, and involved episodes of whiplash-like flagellar bending punctuated by periods of more progressive swimming. The trajectories of the spermatozoa were obviously influenced by the presence of the epithelial surfaces of the oviduct, suggesting that interactions between the spermatozoa and these surfaces could play a role in oviductal sperm transport.

Note:
ACKNOWLEDGMENTS Comments from Dr. J. W. Overstreet are gratefully appreciated.

This article has been cited by other articles:

				X. Druart, J. Cognie, G. Baril, F. Clement, J.-L. Dacheux, and J.-L. Gatti In vivo imaging of in situ motility of fresh and liquid stored ram spermatozoa in the ewe genital tract Reproduction, July 1, 2009; 138(1): 45 - 53. [Abstract] [Full Text] [PDF]

				M. G. O'Rand, E. E. Widgren, S. Beyler, and R. T. Richardson Inhibition of Human Sperm Motility by Contraceptive Anti-Eppin Antibodies from Infertile Male Monkeys: Effect on Cyclic Adenosine Monophosphate Biol Reprod, February 1, 2009; 80(2): 279 - 285. [Abstract] [Full Text] [PDF]

				S. S. Suarez Control of hyperactivation in sperm Hum. Reprod. Update, November 1, 2008; 14(6): 647 - 657. [Abstract] [Full Text] [PDF]

				B. Marquez and S. S. Suarez Bovine Sperm Hyperactivation Is Promoted by Alkaline-Stimulated Ca2+ Influx Biol Reprod, April 1, 2007; 76(4): 660 - 665. [Abstract] [Full Text] [PDF]

				J. Baumber and S. A. Meyers Hyperactivated Motility in Rhesus Macaque (Macaca mulatta) Spermatozoa J Androl, May 1, 2006; 27(3): 459 - 468. [Abstract] [Full Text] [PDF]

				D. E. Buttke, J. L. Nelson, P. N. Schlegel, G. R. Hunnicutt, and A. J. Travis Visualization of GM1 with Cholera Toxin B in Live Epididymal Versus Ejaculated Bull, Mouse, and Human Spermatozoa Biol Reprod, May 1, 2006; 74(5): 889 - 895. [Abstract] [Full Text] [PDF]

				B. Marquez and S. S. Suarez Different Signaling Pathways in Bovine Sperm Regulate Capacitationand Hyperactivation Biol Reprod, June 1, 2004; 70(6): 1626 - 1633. [Abstract] [Full Text] [PDF]

				A. E. Carlson, R. E. Westenbroek, T. Quill, D. Ren, D. E. Clapham, B. Hille, D. L. Garbers, and D. F. Babcock CatSper1 required for evoked Ca2+ entry and control of flagellar function in sperm PNAS, December 9, 2003; 100(25): 14864 - 14868. [Abstract] [Full Text] [PDF]

				R. M. Turner Tales From the Tail: What Do We Really Know About Sperm Motility? J Androl, November 1, 2003; 24(6): 790 - 803. [Full Text] [PDF]

				H.-C. Ho and S. S. Suarez Characterization of the Intracellular Calcium Store at the Base of the Sperm Flagellum That Regulates Hyperactivated Motility Biol Reprod, May 1, 2003; 68(5): 1590 - 1596. [Abstract] [Full Text] [PDF]

				H.-C. Ho and S. S. Suarez An Inositol 1,4,5-Trisphosphate Receptor-Gated Intracellular Ca2+ Store Is Involved in Regulating Sperm Hyperactivated Motility Biol Reprod, November 1, 2001; 65(5): 1606 - 1615. [Abstract] [Full Text] [PDF]

				S. Green and S. Fishel Morphology comparison of individually selected hyperactivated and non-hyperactivated human spermatozoa Hum. Reprod., January 1, 1999; 14(1): 123 - 130. [Abstract] [Full Text] [PDF]