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Biology of Reproduction, Vol 23, 181-187, Copyright © 1980 by Society for the Study of Reproduction
1 Instituto de Biología y Medicina Experimental,
Laboratorio de Esteroides, Obligado 2490,
1428 Buenos Aires, Argentina Spermatozoa undergo a series of modifications during their transit through the epididymis
which lead to the acquisition of fertilizing capacity. The rat epididymis produces and secretes
specific proteins (SEP) which interact with the maturing spermatozoa and might be involved in the
maturation process. In the present study we used a micro-complement fixation technique to determine the concentration of SEP in the caput, corpus and cauda epididymides and the ratio SEP/spermatozoa in each
segment. The highest amounts of SEP were found in the caput and cauda regions (10.42 x 103 and
9.69 x 103 U.SEP/mg tissue, respectively). The ratio SEP/spermatozoa was highest in the caput
(85.5 U.SEP/103 spermatozoa) and decreased towards the cauda. The main site of synthesis of SEP was determined after isolation of the different segments and
by the de novo synthesis of SEP and found to be the caput region. Immunofluorescence revealed
that spermatozoa become coated with SEP as they leave the initial segment and remain coated
throughout the organ.
Accepted on April 1, 1980
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