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Biology of Reproduction, Vol 23, 231-235, Copyright © 1980 by Society for the Study of Reproduction

Biochemical and Structural Differences Between Porcine Ovarian Follicular and Luteal Mitochondria

MICHAEL D. CAMPBELL 1, MICHAEL A. NEYMARK 2, PAMELA K. HILL 3, MICHAEL M. ROTHKOPF 3, , and MICHAEL J. DIMINO 2

1 Department of Research, Sinai Hospital of Detroit, Detroit, Michigan 48235
2 Department of Biochemistry, Eastern Virginia Medical School, Norfolk, Virginia 23501
3 Department of Anatomy, Eastern Virginia Medical School, Norfolk, Virginia 23501


Mitochondria from intact follicles (6-10 mm) and corpora lutea of porcine ovaries were isolated and compared biochemically and structurally. With succinate as substrate, luteal mitochondria consumed oxygen at a rate four times greater than did follicular mitochondria. However, the ADP:O ratio (moles of ADP converted to ATP per atom of oxygen) was significantly lower in luteal mitochondria. Follicular mitochondria synthesized 8.9 µmoles ATP/mg protein/2 h, whereas luteal mitochondria synthesized 21.2 µmoles/mg protein/2 h. Also, luteal mitochondria converted five times more [4-14C]-cholesterol to [4-14C]-pregnenolone than did follicular mitochondria. The amounts of cytochrome aa3 in follicular and luteal mitochondria were 0.052 and 0.15 nmoles/ mg protein, respectively, while the amounts of cytochrome P-450 were 0.011 and 0.21 nmoles/mg protein. Electron microscopic examination of nonsectioned isolated samples showed that luteal mitochondria were significantly larger than follicular mitochondria, It is concluded that luteal mitochondria are more active in steroidogenesis and energy production than are follicular mitochondria. These higher levels of activity in luteal mitochondria probably result, in part, from the presence of additional functional components as indicated by increases in mitochondrial size and amounts of cytochrome aa3 and cytochrome P-450.

Note:
ACKNOWLEDGMENTS This research was supported by NSF Grant PCM 7905425. We wish to thank Frederick and Herrud, Inc., Detroit, MI and ITT Gwaltney, Inc., Smithfield, VA for their generosity in supplying us with porcine ovaries. Also, the technical assistance of Sheila A. Rosenfield-Wessels is acknowledged.

Submitted on February 20, 1980
Accepted on April 29, 1980






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Copyright © 1980 by the Society for the Study of Reproduction.