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Biology of Reproduction, Vol 23, 243-252, Copyright © 1980 by Society for the Study of Reproduction
1 Biology Department,
University of California,
La Jolla, California 92093 Two cell lines have been isolated from testis of immature BALB/c mice. These lines have each
been cloned several times and have been established in culture for over 2 years. The cells are
epithelial in appearance and do not form tumors when injected into BALB/c — nu/nu mice. The
cell lines can be said to be two distinct cell types on the basis of their morphology, hormone
response, responses to gonadotropins, and metabolism of steroids. One line (TM4) has been tentatively identified as being of Sertoli cell origin, since it shows a
growth response to purified ovFSH and an increase in cAMP in the presence of FSH but not LH.
The other line (TM3), believed to be Leydig cells, is not growth-stimulated by either LH or FSH,
shows an increase in cAMP in the presence of LH, and a change in the metabolism of [14C]-cholesterol in the presence of LH.
Note:
ACKNOWLEDGMENTS
I wish to gratefully acknowledge the technical
assistance of Ms. Penelope Roberts. I would also like
to thank Dr. Gordon Sato for stimulating discussions.
This research was supported by NIH Postdoctoral
Training Grant No. 1 F22 CA00821-01 and Grant No.
5-RO1-GM17019-07 (to Dr. G. H. Sato).
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|
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|
|
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