Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by ATKINS, D. T.
Right arrow Articles by FLEEGER, J. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by ATKINS, D. T.
Right arrow Articles by FLEEGER, J. L.
Agricola
Right arrow Articles by ATKINS, D. T.
Right arrow Articles by FLEEGER, J. L.

Biology of Reproduction, Vol 23, 317-323, Copyright © 1980 by Society for the Study of Reproduction

Progesterone-Receptor Complex in the Nuclear Fraction of the Bovine Endometrium

D. T. ATKINS 1, D. C. KRAEMER 2, P. G. HARMS 1, , and J. L. FLEEGER 1

1 Department of Animal Science, Texas Agricultural Experiment Station, Texas A&M University College Station, Texas 77843
2 Department of Veterinary Physiology and Pharmacology, Texas Agricultural Experiment Station, Texas A&M University College Station, Texas 77843


A nuclear exchange assay was utilized to characterize and quantitate the progesterone-receptor complex (PR) of the bovine endometrium. Fifteen Bos taurus cows were observed twice daily for estrus and were assigned to experimental groups at the time of estrus. Endometrium was removed on the day of estrus, Day 4, Day 10 or 11, and Day 18 of the estrous cycle. Scatchard analysis of the nuclear exchange assay resulted in a nonlinear fit which could be linearized by the inclusion of 100-fold excess of cortisol. The number of binding sites per endometrial cell from the uterine horn ipsilateral to the corpus luteum was 34,900 ± 2900 and from the contralateral horn it was 17,000 ± 4400 (P<0.02). Data on the nuclear PR varied between individuals at estrus, Day 4 and Day 18 of the cycle.

Note:
ACKNOWLEDGMENTS The authors are grateful to Drs. Marian Walters and James Clark, Department of Cell Biology, Baylor College of Medicine, Houston, TX, for their technical advice on the nuclear exchange assay. The authors would like to express their appreciation to Ms. Nancy Howard for her assistance in the preparation of this manuscript.

Submitted on October 23, 1979
Accepted on May 26, 1980






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the Society for the Study of Reproduction.