Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 23, 331-335, Copyright © 1980 by Society for the Study of Reproduction

Identification of Riboflavin in Porcine Uterine Secretions

R. J. MOFFATT 1, FINNIE A. MURRAY 1, A. P. GRIFO JR. 1, L. W. HAYNES 2, J. E. KINDER 1, , and G. R. WILSON 3

1 Department of Animal Science, Ohio Agricultural Research and Development Center, Wooster, Ohio 44691
2 Department of Chemistry, College of Wooster, Wooster, Ohio 44691
3 Department of Animal Science, The Ohio State University, Columbus, Ohio 43210


Dr. F. A. Murray, Department of Animal Science, OARDC, Wooster, OH 44691.

A yellow, dialyzable, hydrophilic chromophore is present in the uterine lumen of pigs on Day 8 of the estrous cycle. Microbiological assays, thin layer chromatography, high performance liquid chromatography, uv-visible spectra, and infrared spectra all provide evidence that the yellow substance is riboflavin. The quantity of riboflavin in the uterine secretions was measured by the microbiological assay to be 100.3 ± 16.7 µg of riboflavin per uterine flushing.

Note:
ACKNOWLEDGMENTS We thank Ms. Jackie Zurcher of the College of Wooster for the determination of infrared spectra.

Submitted on February 15, 1980
Accepted on May 29, 1980






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Copyright © 1980 by the Society for the Study of Reproduction.