Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 23, 453-459, Copyright © 1980 by Society for the Study of Reproduction

Temporal Aspects of Rabbit Uterine Vascular and Decidual Responses to Blastocyst Stimulation

PAULA C. HOOS 1, and LOREN H. HOFFMAN 1

1 Department of Anatomy, Vanderbilt University School of Medicine, Nashville, Tennessee 37232


Measurements were made of the permeability of rabbit uterine blood vessels to protein-bound Evans blue (EB) dye at implantation sites relative to nonimplant regions of the uterus. Results, expressed as a permeability ratio (implant EB/nonimplant EB), showed that a vascular response was present at some implant sites as early as 6.5 days after mating, while higher permeability ratios (>3.0) were obtained at 7 and 8 days. Assay of tissue water (edema) in implantation sites correlated well with changes in the degree of bluing. On Day 6.5 or 7, several intact blastocysts from sites exhibiting bluing were flushed from the uterus. Light microscopic analysis of the blastocysts revealed intact blastocyst coatings suggesting that the vascular response was initiated prior to direct attachment of trophoblast to uterine epithelium in rabbits. In another experiment, the duration of blastocyst-uterine contact required for maximal stimulation of decidual development was assessed by destruction of blastocysts at 6.5, 6.75, 7, 7.5, or 8 days after mating. Maximal decidual development, measured on Day 14, was obtained only in uteri of females where blastocysts were destroyed at, or after, 7.5 days. Thus, for most females a period of blastocyst-uterine contact approaching 24 h is required for full commitment to decidual development.

Note:
ACKNOWLEDGMENTS We wish to express our appreciation to Dr. Margaret Ward Orsini for her encouragement and to Dr. Gary Olson and Dr. Orsini for reading the manuscript. We also thank Mrs. Virginia Winfrey for technical assistance, Dr. G. Rodman Davenport for assistance in performing the Evans blue determination, and Ms. Brenda Lair for typing the manuscript. This work was supported by NIH Training Grant HD-07043 (P.C.H.) and Research Grant HD-05942 (L.H.H.).

Submitted on April 7, 1980
Accepted on June 27, 1980




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