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Biology of Reproduction, Vol 23, 570-576, Copyright © 1980 by Society for the Study of Reproduction

Biochemical Properties of Equine Chorionic Gonadotropin from Two Different Pools of Pregnant Mare Sera

BHARAT B. AGGARWAL 1, SUSAN WALKER FARMER 1, HAROLD PAPKOFF 2, , and GEORGE E. SEIDEL Jr. 3

1 Hormone Research Laboratory, University of California, San Francisco, California 94143
2 Hormone Research Laboratory, and Reproductive Endocrinology Center, University of California, San Francisco, California 94143
3 Animal Reproduction Laboratory, Colorado State University, Fort Collins, Colorado 80523


Dr. Harold Papkoff, Hormone Research Laboratory, 1088 HSW, University of California, San Francisco, CA 94143.

Equine chorionic gonadotropin (eCG, PMSG) has been isolated from two different pools (high and low titer) of pregnant mare sera using the methodology previously employed for the isolation of eCG. Gonadotropin from both pools of sera exhibit a similar apparent molecular weight (Ve/Vo = 1.2-1.3) on gel exclusion chromatography. Chemically, the two types of eCG are different in both amino acid and carbohydrate composition. Low titer serum eCG (eCGLT) has significantly less carbohydrate (25%) than does high titer gonadotropin (eCGHT) (46%). Phenylalanine and serine were detected as major amino termini in high titer eCG in comparison to phenylalanine and leucine in low titer gonadotropin. Immunologically, high titer eCG is considerably more active (9750 IU/mg) than low titer eCG (2850 IU/mg). Both in vitro and in vivo bioassays for LH and FSH also show that purified low titer eCG has a lower specific activity (1500-5700 IU/mg) than does high titer serum gonadotropin (3450-13,050 IU/mg). Both biologically and chemically, low titer eCG more closely resembles eCG isolated from trophoblastic tissue or from equine chorionic cell culture medium, whereas high titer eCG resembles eCG previously isolated from serum. Our results demonstrate the natural existence in pregnant mare serum of different forms of eCG, which differ not only chemically but in biological and immunological potency.

Note:
ACKNOWLEDGMENTS We wish to thank Drs. Ted Hayashida and Selna Kaplan and Mrs. Eleanor Rowley for assistance with the radioimmunoassays, Mr. J. D. Nelson and Mr. K. Hoey for expert technical assistance, and Dr. G. D. Niswender for testosterone antibody. The encouragement and interest of Professor Choh Hao Li in these studies is appreciated. This work was supported in part by a grant from NIH (HD-05722). B. B. A. was a Fellow of the Population Council.

Submitted on March 3, 1980
Accepted on July 15, 1980






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Copyright © 1980 by the Society for the Study of Reproduction.