Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by PETERSON, R.
Right arrow Articles by FREUND, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by PETERSON, R.
Right arrow Articles by FREUND, M.
Agricola
Right arrow Articles by PETERSON, R.
Right arrow Articles by FREUND, M.

Biology of Reproduction, Vol 23, 637-645, Copyright © 1980 by Society for the Study of Reproduction

Evaluation of the Purity of Boar Sperm Plasma Membranes Prepared by Nitrogen Cavitation

RUDOLPH PETERSON 1, LONNIE RUSSELL 1, DONNA BUNDMAN 1, , and MATTHEW FREUND 1

1 School of Medicine and Department of Physiology, Southern Illinois University, Carbondale, Illinois 62901


Quantitative electron microscopy is used to provide new information regarding the purity and the amounts of plasma membrane originating from various regions of the surface of the boar spermatozoon when such membranes are prepared by nitrogen cavitation and sucrose density centrifugation. These data show that the plasma membranes originate primarily from the head of the spermatozoon; they also show that contamination of these plasma membranes by acrosomal and mitochondrial membranes is negligible. Immunolocalization, using plasma membrane vesicles and divalent IgG directed against plasma membrane antigens, shows that plasma membrane vesicles bind extensively to intact sperm plasma membranes but rarely bind to the outer acrosomal membrane. These new data, taken together with that which has been previously reported, indicate that high pressure gas cavitation provides a simple means for obtaining undamaged, highly purified sperm plasma membranes.

Note:
ACKNOWLEDGMENTS The use of the electron microscope facilities at the Springfield campus of Southern Illinois University was greatly appreciated. The excellent technical assistance of Ms. Terri Russell, Mr. Ray Venezia, and Mr. S. Usaha is acknowledged. This study was supported by NIH Grant HD 10947.

Submitted on March 3, 1980
Accepted on July 16, 1980




This article has been cited by other articles:


Home page
 ReproductionHome page
K E Waterhouse, P O Hofmo, A Tverdal, and R R Miller Jr
Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm.
Reproduction, May 1, 2006; 131(5): 887 - 894.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A. Kurz, D. Viertel, A. Herrmann, and K. Muller
Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction
Reproduction, November 1, 2005; 130(5): 615 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. M. Flesch, W. F. Voorhout, B. Colenbrander, L. M.G. van Golde, and B. M. Gadella
Use of Lectins to Characterize Plasma Membrane Preparations from Boar Spermatozoa: A Novel Technique for Monitoring Membrane Purity and Quantity
Biol Reprod, December 1, 1998; 59(6): 1530 - 1539.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1980 by the Society for the Study of Reproduction.