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Biology of Reproduction, Vol 23, 663-668, Copyright © 1980 by Society for the Study of Reproduction

Blastocyst Implantation in the Rat and the Immunohistochemical Distribution and Rate of Synthesis of Uterine Lysosomal Cathepsin D

SUDHA ELANGOVAN 1, and BRUCE C. MOULTON 1

1 Department of Obstetrics and Gynecology, Department of Biological Chemistry, and Department of Physiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45267


Major concentrations of the lysosomal protease, cathepsin D, identified by immunohistochemical staining were observed in glandular and luminal epithelial cells in cross sections of uteri of pregnant rats. Blastocyst implantation decreased the level of cathepsin D observed in the luminal epithelium on Day 5 of pregnancy. The rate of synthesis of the enzyme protein in uterine segments containing implantation sites was approximately half the rate measured in interimplantation uterine segments. These data suggest that deterioration and eventual removal of uterine epithelial cells during blastocyst implantation involve epithelial cell lysosomes and their content of cathepsin D.

Note:
ACKNOWLEDGMENTS The authors would like to thank Dr. James Lessard for his assistance with immunohistochemical staining techniques and fluorescence photography. We thank Dr. Roger Granschow for assistance with the methods of measuring amino acid incorporation into immunoprecipitable protein. Carol E. Bates and Beth B. Koenig provided excellent technical assistance. This research was supported by grants from NIH (HD-07255 and HD-10721).

Submitted on April 21, 1980
Accepted on July 9, 1980




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G. Song, T. E. Spencer, and F. W. Bazer
Cathepsins in the Ovine Uterus: Regulation by Pregnancy, Progesterone, and Interferon Tau
Endocrinology, November 1, 2005; 146(11): 4825 - 4833.
[Abstract] [Full Text] [PDF]


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Copyright © 1980 by the Society for the Study of Reproduction.