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Biology of Reproduction, Vol 23, 1075-1085, Copyright © 1980 by Society for the Study of Reproduction
1 Pregnancy Research Branch,
National Institute of Child Health and Human Development,
National Institutes of Health,
Bethesda, Maryland 20205 The interactions of mammalian sperm with the zona pellucida of eggs, living or fixed with
glutaraldehyde, have been investigated in three rodent species. Qualitative observations of spermegg interactions in both homologous and heterologous mixtures of guinea pig, mouse, and
hamster gametes revealed that 1) sperm initially attach in a loose reversible manner to homologous
eggs and subsequently, stable irreversible binding is observed; 2) mouse and hamster sperm attach
and bind to both mouse and hamster eggs with similar affinities; and 3) guinea pig sperm attach
and bind only to homologous eggs, and attachment or binding to heterologous eggs is not observed. Quantitative assessment of sperm binding to mouse and hamster eggs gave similar results. When
the kinetics of mouse, hamster, and guinea pig sperm binding to eggs was measured using a 100-fold variation in sperm concentrations, mouse and hamster sperm showed nonspecific binding,
whereas guinea pig sperm did not bind. Similar results were obtained when sperm binding was
studied with living or fixed eggs.
Note:
ACKNOWLEDGMENTS
The authors gratefully acknowledge the expert
technical assistance of Mr. C. K. Turner and Mr. D.
Barber. We also thank Ms. L. Baldwin for her help in
preparing the manuscript.
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