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Biology of Reproduction, Vol 24, 234-240, Copyright © 1981 by Society for the Study of Reproduction

Bovine Sperm Forward Motility Protein: Binding to Epididymal Spermatozoa

TED S. ACOTT 1, and DALE D. HOSKINS 1

1 Department of Reproductive Physiology, Oregon Regional Primate Research Center, Beaverton, Oregon 97006


Immature spermatozoa from the bovine caput epididymis develop an uncoordinated thrashing motion in response to an elevation in cyclic AMP levels and convert to strong progressive motility upon the subsequent addition of bovine forward motility protein. We show that this protein activates caput sperm in a switchlike, all-or-none manner and that the activation is not easily reversible.

We also present evidence that the forward motility protein binds to caput sperm as they pass through the epididymis. First, the continued presence of free forward motility protein is not necessary. Second, the activation does not involve a simple enzymatic mechanism. Third, the forward motility protein can be depleted from solution by caput, but not caudal, epididymal sperm. Fourth, forward motility protein can be extracted from caudal, but not caput, epididymal sperm with Triton X-100.

Note:
ACKNOWLEDGMENTS We are indebted to Howard Brandt, Dave Battalia, Don Stephens, and Lisa Critchlow for discussion of the experiments and to Beth Jeppesen for patient assistance with the manuscript. The work described in this article, Publication No. 1117 of the Oregon Regional Primate Research Center, was supported by NIH Grants RR-00163, HD-05969, and HD-11982.

Submitted on July 28, 1980
Accepted on October 14, 1980




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N. P. Haden, J. R. Hickox, C. Scott Whisnant, and D. M. Hardy
Systematic Characterization of Sperm-Specific Membrane Proteins in Swine
Biol Reprod, December 1, 2000; 63(6): 1839 - 1847.
[Abstract] [Full Text]


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Copyright © 1981 by the Society for the Study of Reproduction.