Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by HUNZICKER-DUNN, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by HUNZICKER-DUNN, M.
Agricola
Right arrow Articles by HUNZICKER-DUNN, M.

Biology of Reproduction, Vol 24, 279-286, Copyright © 1981 by Society for the Study of Reproduction

Rabbit Follicular Adenylyl Cyclase Activity. II. Gonadotropin-Induced Desensitization in Granulosa Cells and Follicle Shells

MARY HUNZICKER-DUNN 1

1 Department of Biochemistry, Northwestern University Medical School, Chicago, Illinois 60611


Previous studies reported a simultaneous decline in luteinizing hormone (LH)-and follicle stimulating hormone(FSH)-stimulated adenylyl cyclase (AC) activities in rabbit follicles exposed in vivo to a coital-induced surge of LH or a bolus of human chorionic gonadotropin (hCG) (Hunzicker-Dunn and Birnbaumer, 1976). In these studies, FSH-stimulable AC activity was measured using an FSH preparation which contained sim1% LH (Reichert and Wilhelrni, 1973). In an effort to evaluate more precisely the role of FSH in follicular function in rabbits, AC activity has been reevaluated using a highly purified fraction of FSH to measure FSH-stimulated AC activity. LH- and FSH-stimulable AC activities were measured both in rabbit follicular granulosa cells and in thecal cell-predominated follicle shells at various times after mating or the injection of an ovulatory concentration of hCG.

In follicle shells, LH-stimulated AC rapidly fell during the first hour after mating or hCG injection while FSH-stimulated AC did not change. Both AC parameters declined to near basal levels by 7 h.

The rate of AC desensitization was markedly different in granulosa cells. No significant changes in LH- or FSH-stimulated AC activities occurred during the first 2 h following either the endogenous surge of LH or the exogenous bolus of hCG, but by 4.5 h gonadotropin-stimulated AC activities were significantly (P<0.001) reduced. However, when isolated granulosa cells were exposed in vitro for 60 min to either LU or FSH, both the homologous and the heterologous gonadotropin-stimulated AC activities, respectively, were reduced by 50%.

These results demonstrate different rates of AC desensitization in follicle shells to LH and FSH when a purified fraction of FSH is used to measure FSH-stimulated AC activity. They also reveal markedly different rates of AC desensitization to gonadotropins in vivo between granulosa cells and thecal shells. Indirect evidence suggests that the retarded rate of AC desensitization in granuloss cells may be attributable to the avascularity of this follicular compartment.

 Submitted on June 9, 1980
Accepted on October 30, 1980




This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
R. D. Horvat, B. G. Barisas, and D. A. Roess
Luteinizing Hormone Receptors Are Self-Associated in Slowly Diffusing Complexes during Receptor Desensitization
Mol. Endocrinol., April 1, 2001; 15(4): 534 - 542.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
A. P. N. Themmen and I. T. Huhtaniemi
Mutations of Gonadotropins and Gonadotropin Receptors: Elucidating the Physiology and Pathophysiology of Pituitary-Gonadal Function
Endocr. Rev., October 1, 2000; 21(5): 551 - 583.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
S. Mukherjee, K. Palczewski, V. V. Gurevich, and M. Hunzicker-Dunn
beta -Arrestin-dependent Desensitization of Luteinizing Hormone/Choriogonadotropin Receptor Is Prevented by a Synthetic Peptide Corresponding to the Third Intracellular Loop of the Receptor
J. Biol. Chem., May 7, 1999; 274(19): 12984 - 12989.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. M. Rajagopalan-Gupta, S. Mukherjee, X. Zhu, Y.-K. Ho, H. Hamm, M. Birnbaumer, L. Birnbaumer, and M. Hunzicker-Dunn
Roles of Gi and Gq/11 in Mediating Desensitization of the Luteinizing Hormone/Choriogonadotropin Receptor in Porcine Ovarian Follicular Membranes
Endocrinology, April 1, 1999; 140(4): 1612 - 1621.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Mukherjee, K. Palczewski, V. Gurevich, J. L. Benovic, J. P. Banga, and M. Hunzicker-Dunn
A direct role for arrestins in desensitization of the luteinizing hormone/choriogonadotropin receptor in porcine ovarian follicular membranes
PNAS, January 19, 1999; 96(2): 493 - 498.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. L. G. Lamm, R. M. Rajagopalan-Gupta, and M. Hunzicker-Dunn
Epidermal Growth Factor-Induced Heterologous Desensitization of the Luteinizing Hormone/Choriogonadotopin Receptor in a Cell-Free Membrane Preparation Is Associated with the Tyrosine Phosphorylation of the Epidermal Growth Factor Receptor
Endocrinology, January 1, 1999; 140(1): 29 - 36.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1981 by the Society for the Study of Reproduction.