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Biology of Reproduction, Vol 24, 455-459, Copyright © 1981 by Society for the Study of Reproduction

Utero-ovarian Vein Blood Collection by the Use of a Laparoscope in the Baboon

ABUBAKAR A. SHAIKH 1, SHAMIM A. SHAIKH 1, , and CORNELIO L. CELAYA 1

1 Southwest Foundation for Research and Education, San Antonio, Texas 78284


A technique for the collection of utero-ovarian vein blood in the baboon is described. It makes use of a laparoscope and transabdominal puncture with a long blood-collection needle. This technique enables study of changes in the gonadal hormone output before these hormones become diluted in the peripheral circulation. It is expected that levels of hormones in the utero-ovarian vein blood would be more indicative of the hormonal milieu in the ovarian compartment than are the peripheral levels. Thus, this method provides a mechanism for longitudinal studies of dynamics of the ovarian hormonal output without resorting to repeated laparotomies.

Note:
ACKNOWLEDGMENTS The authors would like to thank Ignacio Gomez for his skillful technical assistance. We also thank Dr. J. W. Goldzieher for his critical comments and Ms. Jo Fletcher for her editorial assistance. The blood collection technique described was first developed using cynomolgus monkeys, supported by NICHD grant HD09964. This study was supported by NICHD grant HD15300.

Submitted on January 30, 1980
Accepted on November 10, 1980






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Copyright © 1981 by the Society for the Study of Reproduction.