Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 24, 955-961, Copyright © 1981 by Society for the Study of Reproduction

Control of Sertoli Cell Response to FSH: Regulation by Homologous Hormone Exposure

GARY L. FRANCIS 1, TERI J. BROWN 1, , and BARRY B. BERCU 1

1 Neonatal and Pediatric Medicine Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20205


The effect of in vitro exposure to FSH on subsequent FSH binding and stimulation of short-term rat Sertoli cell cultures was investigated. Cultures were exposed 6 h to FSH (0.05, 0.1, 1.0, or 5.0 µg/ml), washed, and incubated for an additional 1, 2, 4, or 6 days at which times FSH binding, FSH-stimulated cAMP generation, and FSH-stimulated aromatization of testosterone (T) to estradiol (E2) were determined.

The initial effect of exposure to high concentrations of FSH (1.0-5.0 µg/ml) was a dose-related decrease in both hormone binding and cAMP response. In contrast, a dose-related increase in T aromatization occurred. By 4-6 days after FSH exposure, hormone binding had started to increase, and cAMP response had either returned to or was significantly increased above control values while T aromatization remained elevated without any additional increase.

The initial effect after exposure to low concentrations of FSH (0.05-0.1 µg/ml) was an intermediate decrease in hormone binding with no significant change in either cAMP generation or T aromatization. By 4-6 days after FSH exposure, hormone binding and cAMP response were significantly increased above control values. T aromatization did not change.

These findings suggest that FSH regulates Sertoli cell response to subsequent FSH stimulation apparently at two independent levels: one related to hormone binding and cAMP response, and the second related to the aromatization of T.

Note:
ACKNOWLEDGMENTS The authors wish gratefully to acknowledge the advice of Dr. Griff Ross; the editorial assistance of Dr. Marvin Cornblath and Dr. Griff Ross; Ms. Kitty Kunkle and Ms. Deborah Kirk for their excellent secretarial support; and the NPA of the NIAMDD for the purified gonadotropins NIH-FSH-S-13 and ovine NIH-LH-21.

Submitted on November 7, 1980
Accepted on January 21, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.