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Biology of Reproduction, Vol 25, 110-114, Copyright © 1981 by Society for the Study of Reproduction

Influence of Temperature on Amino Acid Transport by Round Spermatids from Rat Testis

PETER F. HALL 1, MASATOSHI MITA 1, , and MASAHISA NAKAMURA 1

1 The Worchester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545


Transport of [14C]-agr-aminoisobutyric acid in vitro by round spermatids from rat testis was found to be more rapid at 34°C than at 37°C and more rapid at 37°C than at 40°C. The difference between values at 34°C and 37°C could be reversed by changing the spermatids from 37°C to 34°C at various times during a total incubation period of 1 h. The effect of 37°C was exerted upon Km for the transport process; Vmax was unaltered. Inhibition by 37°C relative to 34°C was exerted on influx; efflux was the same at both temperatures. A fraction of cells from rat testis enriched in spermatocytes but free from spermatids showed greater transport at 37°C than at 34°C.

Of the three systems for transport of amino acids (A, ASC, and L), the temperature effect was observed only with the A system. These results indicate that one metabolic change produced by body temperature (37°C) as opposed to scrotal temperature (34°C) is inhibition of amino acid transport.

Note:
ACKNOWLEDGMENTS The authors are grateful to Dr. Grover C. Stephens, Dept. of Developmental and Cell Biology, University of California Irvine, for helpful discussions during the preparation of this manuscript.

Submitted on December 24, 1980
Accepted on March 17, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.