Biology of Reproduction, Vol 25, 191-195, Copyright © 1981 by Society for the Study of Reproduction

Prostaglandin E₂ Stimulates Cumulus Expansion and Hyaluronic Acid Synthesis by Cumuli Oophori Isolated from Mice

¹ The Jackson Laboratory, Bar Harbor, Maine 04609

The effect of prostaglandins on the stimulation of cumulus expansion and hyaluronic acid synthesis by cumuli oophori isolated from mouse Graafian follicles was determined. It was found that prostaglandin E₂ (PGE₂), but not prostaglandin F₂ (PGF₂), stimulated cumulus expansion and hyaluronic acid synthesis by the isolated cumuli oophori. Indomethacin, a prostaglandin synthetase inhibitor, did not block cumulus expansion or hyaluronic acid synthesis induced by follicle stimulating hormone (FSH). In addition, when the isolated cumuli oophori were incubated in medium containing maximally stimulating concentrations of both PGE₂ and FSH, significantly more hyaluronic acid was synthesized than in response to either the PGE₂ or the FSH alone. Therefore, PGE₂ is probably not an obligatory intermediate in the FSH-stimulation of cumulus expansion by isolated cumuli oophori. It is suggested that PGE₂ may play a role in the indirect stimulation of cumulus expansion by luteinizing hormone in vivo.

Note:
ACKNOWLEDGMENTS This research was supported by a grant from NSF (PCM 79-10618). I thank Pat Ward, Rachel Snow, and Avis Silva for their excellent assistance in this research. I am grateful to Dr. A. F. Parlow and the Rat Pituitary Distribution Program of the NIAMDD for generously providing the rFSH. The Jackson Laboratory is fully accredited by the American Association for Accreditation of Laboratory Animal Care.

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