Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 25, 56-64, Copyright © 1981 by Society for the Study of Reproduction

Prostaglandin Formation by the Sheep Embryo and Endometrium as an Indication of Maternal Recognition of Pregnancy

GEORGE J. MARCUS 1

1 Animal Research Institute, Agriculture Canada, Ottawa, Ontario, K1A 0C6, Canada


Enzymatically dispersed ovine endometrial cells of Days 12, 15, and 20 of pregnancy formed prostaglandins from radioactively labeled arachidonic acid in vitro. The major products were prostacyclin, PGE2, and PGF2agr. Cells of Day 15 of pregnancy were the most active. Endometrial cells from nonpregnant sheep at Days 12 or 15 of the estrous cycle were unable to form prostaglandins from labeled precursor under the same conditions although the cells concentrated substrate from the incubation medium. In contrast, homogenized endometrium actively metabolized arachidonic acid to prostaglandins.

Intact embryos of Days 12 and 15 of gestation and cells of chorionic membranes of Day 20 of pregnancy also produced prostacyclin, PGE2, and PGF2agr from labeled arachidonate in vitro.

The findings suggest that maternal recognition of pregnancy occurs by Day 12 and involves functional reorganization of the endometrial enzymatic apparatus to initiate production of prostanoids which may prevent luteal regression and stimulate luteal function in concert with embryonic products.

Note:
ACKNOWLEDGMENTS We are grateful to Mr. J. Wittwer and Mr. B. Durnford for their excellent technical assistance and to Mr. D. Ostapyk for animal care and preparation.

Submitted on October 29, 1980
Accepted on March 3, 1981




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Z Cheng, E L Sheldrick, E Marshall, D C Wathes, D R E Abayasekara, and A P F Flint
Control of cyclic AMP concentration in bovine endometrial stromal cells by arachidonic acid
Reproduction, May 1, 2007; 133(5): 1017 - 1026.
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Copyright © 1981 by the Society for the Study of Reproduction.