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Biology of Reproduction, Vol 25, 290-294, Copyright © 1981 by Society for the Study of Reproduction

Effect of an Aromatase Inhibitor (4-Acetoxy-4-androstene-3,17-dione) on the Stimulatory Action of Luteinizing Hormone on Estradiol-17beta Synthesis by Rat Preovulatory Follicles in vitro

GARETH EVANS 1, PETER C. K. LEUNG 1, ANGELA M. H. BRODIE 2, , and DAVID T. ARMSTRONG 1

1 MRC Group in Reproductive Biology, Department of Obstetrics and Gynaecology and Department of Physiology, University of Western Ontario, London, Ontario, Canada N6A 5A5
2 Department of Pharmacology and Experimental Therapeutics, University of Maryland Medical School, Baltimore, Maryland 21201


The effect of 4-acetoxy-4-androstene-3,17-dione (4-acetoxy-A), an inhibitor of aromatase activity, on the steriodogenic response of preovulatory rat follicles to luteinizing hormone (LH) in vitro was examined. In addition to markedly inhibiting the cumulative secretion of estradiol-17beta, 4-acetoxy-A significantly increased the accumulation of testosterone, whether or not LH was present during culture; addition of exogenous estradiol to culture media containing LH and 4-acetoxy-A partially inhibited testosterone accumulation. Furthermore, 4-acetoxy-A enhanced progesterone accumulation, particularly when the follicles were stimulated by LH. These latter effects did not appear to be explainable solely on the basis of substrate accumulation due to inhibition of aromatization by 4-acetoxy-A. Though 4-acetoxy-A may have direct effects on steroidogenesis, these data support the hypothesis that intrafollicular estrogen levels play a physiological role in modulating steroidogenic responses of the follicle to LH.

Note:
ACKNOWLEDGMENTS We thank the Hormone Distribution Office, NIH, Bethesda, MD, for donating the luteinizing hormone (NIH-LH-B8), and Dr. T. G. Kennedy, University of Western Ontario, for advice with statistical analysis.

Submitted on February 29, 1980
Accepted on April 9, 1981




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Copyright © 1981 by the Society for the Study of Reproduction.