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Biology of Reproduction, Vol 25, 342-348, Copyright © 1981 by Society for the Study of Reproduction

Transfer of Steroids Across the Hamster Blood Testis and Blood Epididymal Barriers

T. T. TURNER 1, R. C. COCHRAN 1, , and S. S. HOWARDS 1

1 Department of Urology and Department of Physiology, University of Virginia Medical School, Charlottesville, Virginia 22908 and Department of Population Dynamics, Johns Hopkins University, Baltimore, Maryland 21205


Hamsters were administered i.v. infusions of solutions containing either [3H] testosterone (T), [3H] dihydrotestosterone (DHT), or [3H] progesterone (P). Micropuncture of seminiferous and epididymal tubules and scintillation spectrophotometry of samples was used to determine the concentration of isotope in blood and intraluminal fluid from both types of reproductive tubules. After equilibration, mean isotope concentrations appearing in seminiferous tubule fluid (SNF) were 31%, 24%, and 14% of blood isotope concentrations for [3H] P, [3H] T, and [3H] DHT, respectively. The same figures for cauda epididymidal fluid (CDF) were 33%, 31%, and 20% for [3H] P, [3H] T, and [3H] DHT, respectively. Isotope from [3H] T and [3H] DHT appeared in CDF in significantly greater (P<0.01) concentrations than in SNF. High pressure liquid chromatography (HPLC) of SNF and CDF after systemic infusion of [3H] P, [3H] T, and [3H] DHT was also performed. Isotope collected in SNF during [3H] T infusion chromatographed primarily with T; in the CDF the isotope (73% of total extractable radioactivity) was associated neither with T nor DHT but with an unidentified highly polar compound(s). Similar conversion occurred in CDF when [3H] DHT was the infused steroid. 5agr Androstane-3agr, 17beta diol was the apparent major metabolite of DHT in SNF. Isotope from [3H] P appeared mostly as unidentified highly polar compound in SNF and as [3H] P in CDF. The restricted transport of isotope into both SNF and CDF plus the further metabolism of the steroids infused demonstrate that circulating androgens have limited direct access to the intraluminal compartment of the male hamster reproductive tract.

Note:
ACKNOWLEDGMENTS This investigation was supported by NIH Grant HD90490 and NIH Career Development Award 1-K04-HD00108 (S.S.H.).

Submitted on March 6, 1981
Accepted on April 28, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.