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Biology of Reproduction, Vol 25, 375-384, Copyright © 1981 by Society for the Study of Reproduction

Turkey Prolactin: Purification by Isotachophoresis and Partial Characterization

J. A. PROUDMAN 1, and D. H. CORCORAN 2

1 U.S. Department of Agriculture, SEA-AR, Animal Sciences Institute, Avian Physiology Laboratory, Beltsville, Maryland 20705
2 Department of Biochemistry, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20014


Preparative isotachophoresis (ITP) has been employed in the purification of prolactin from the pituitary gland of the domestic turkey. Prolactin was purified in relatively few steps and required only small quantities of pituitary tissue. The resultant highly purified hormone was recovered in substantially higher yield and with greater biological activity than was turkey prolactin purified by other techniques. Total yield of purified prolactin was 850 mg/kg of wet weight of pituitaries, but aggregation of prolactin into high molecular weight polymers during the purification process reduced the yield of biologically active monomeric prolactin to 446 mg/kg.

Turkey prolactin showed molecular polymorphism during purification and characterization. The hormone was isolated as two peaks from ITP, resolved into at least five bands on isoelectric focusing and disc electrophoresis, and exhibited two molecular forms on SDS electrophoresis. The chemical and biological characteristics of turkey prolactin isolated by ITP were similar, though not identical, to those of turkey and chicken prolactins purified by conventional techniques.

Note:
ACKNOWLEDGMENTS The authors wish especially to thank Dr. G. P. Birrenkott, Clemson University, for performing the crop-sac bioassay, and N. G. Zimmermann and Dr. B. C. Wentworth, University of Wisconsin, for statistical analysis of the bioassay data. The assistance of Dr. H. Opel, S. R. McGuire, and C. L. Avery in collection of pituitary tissue is gratefully acknowledged, as is the invaluable cooperation provided by Wampler Foods, Inc., Hinton, VA. Thanks are given to S. R. McGuire for preparing the figures and to S. R. Trost for technical assistance. Ovine prolactin and rat FSH preparations used in this study were kindly supplied by the NIAMDD, NIH, Bethesda, MD. Turkey LH was provided by Dr. W. H. Burke, University of Minnesota; antiserum to turkey LH was provided by Dr. B. C. Wentworth.

Submitted on December 23, 1980
Accepted on April 8, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.