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Biology of Reproduction, Vol 25, 385-392, Copyright © 1981 by Society for the Study of Reproduction

Evidence for agr-Lactalbumin-like Activity in Reproductive Tract Fluids of the Male Rat

DAVID W. HAMILTON 1

1 Department of Anatomy, University of Minnesota, Minneapolis, Minnesota 55455


Synthesis of lactose by rat RTF galactosyltransferase and a fraction of rat epididymal fluid is described and shown to be similar to the lactose synthetase complex described heretofore only in lactating breast and milk. Partial purification of the agr-lactalbumin-like material on Sephadex G-75 and DEAE-BioGel A yielded two fractions containing material that migrated on SDS-polyacrylamide gels to bands corresponding to molecular weights consistent with those reported for rat milk agr-lactalbumin.

Note:
ACKNOWLEDGMENTS I would like to thank Jovita Baker for her dedicated and expert help in technical aspects of this project, Karen Westphall for collecting the RTF, and Dr. Robert Olson for doing the lysozyme assay. Dr. Robert Sorenson provided helpful comments on the manuscript.

Submitted on February 23, 1981
Accepted on April 9, 1981




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Copyright © 1981 by the Society for the Study of Reproduction.