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Biology of Reproduction, Vol 25, 393-398, Copyright © 1981 by Society for the Study of Reproduction

Uterine Lysosomal Cathepsin D Activity, Rate of Synthesis, and Immunohistochemical Localization Following Initiation of Decidualization in Pseudopregnant Rats

BRUCE C. MOULTON 1, and CAROL BATES INGLE 1

1 Departments of Obstetrics and Gynecology, Biological Chemistry, and Physiology, University of Cincinnati, College of Medicine, Cincinnati, Ohio 45267


Initiation of decidualization in pseudopregnant rats by intrauterine administration of an oil stimulus resulted in significant decreases in the activity of the lysosomal protease, cathepsin D, and significant decreases in the rate of synthesis of the enzyme. Decreases in uterine cathepsin D activity after the decidualizing stimulus required delivery of the stimulus on Day 4 of pseudopregnancy. Major concentrations of cathepsin D protein identified by immunohistochemical staining were observed in glandular and luminal epithelial cells. The accumulation of cathepsin D in the luminal epithelial cells during early pseudopregnancy could provide these cells with the means for cellular autophagic activity and eventual destruction during the uterine decidual response.

Note:
ACKNOWLEDGMENTS The authors would like to thank Dr. James Lessard for his assistance with immunohistochemical staining techniques and fluorescence photography. We thank Dr. Roger Ganschow for assistance with the methods of measuring amino acid incorporation into immunoprecipitable protein. Matthew McBride provided valuable technical assistance. This research was supported by grants from NIH (HD-07255 and HD-10721).

Submitted on December 1, 1980
Accepted on April 13, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.