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Biology of Reproduction, Vol 25, 439-450, Copyright © 1981 by Society for the Study of Reproduction
1 The Population Council,
Center for Biomedical Research,
New York, New York 10021 The effect of active immunization with porcine or rabbit zonae pellucidae on the fertility of
rabbits was studied. A total of 28 rabbits in groups of four were immunized with 1) intact, 2) heat-solubilized or 3) detergent-dissociated (SDS-sodium sulfite-solubilized) porcine or rabbit zonae,
or 4) complete Freund’s adjuvant. Serum antibodies to zona antigens were monitored by immunoelectrophoresis and by radioimmunoassay methods. All of the animals in the groups which were
heteroimmunized with intact or with heat-solubilized porcine zonae were infertile in both term
pregnancy studies or in 4-8-cell embryo recovery studies. Normal ovarian function was interrupted in these animals, since normal ova were not ovulated after hCG treatment. Ovulation appeared normal in animals immunized with detergent-dissociated porcine zonae or with rabbit zonae
in any form. Although fertility was reduced in these latter groups, some animals had normal offspring or normal cleaving embryos even though antibodies to zonae were present in their serum.
These studies demonstrate that 1) heteroimmunization of rabbits with porcine zonae is more
effective in reducing fertility than alloimmunization with rabbit zonae; 2) the reduction of fertility by immunization with zonae is dependent on the state of solubilization of zonae prior to immunization; 3) serum antibodies to some zona antigens can be present which do not inhibit fertilization; and 4) the reason(s) for infertility by active immunization with zona antigens may be other
than (or in addition to) the blockage of sperm binding to the zonae or penetration of the zonae.
We conclude that the rabbit is an excellent model for the comparison of the effects on fertility of
alloimmunization and heteroimmunization with zona antigens.
Note:
ACKNOWLEDGMENTS
The authors wish to thank Dr. M. Gerrity, D.
Ackman, S. Roberts and T. Barnett for their help with
parts of this study. We express our gratitude to the
Rockefeller University Animal Care staff for their
consideration and support in preparing for the fertility
studies and animal autopsies. We thank Dr. C. W.
Bardin for advice on the preparation of this manuscript. This work was supported by grants HD12347-01 and HD13541 from NICHD.
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