| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 25, 683-691, Copyright © 1981 by Society for the Study of Reproduction
1 Department of Physiology,
Ralph L. Smith Research Center,
University of Kansas Medical Center,
Kansas City, Missouri 66103 The interrelationship between follicular and luteal function during pregnancy in the rat was
examined by in vivo and in vitro methods. Corpora lutea (CL) and nonluteal ovarian tissues (NLO)
were removed on Days 2 to 22 (at 2 day intervals) (day sperm-positive = Day 1 of pregnancy), and
incubated separately for 2 h to determine the production rate of steroids. Changes in progesterone
(P), 20 A marked decline in the in vitro production rate of E2 and T in NLO occurred between Days
14 and 18 followed by an abrupt increase on Days 20 and 22. These changes correlated with the
levels of serum LH between Days 14 and 22. Serum FSH also declined on Day 16 but returned to
basal levels by Day 18. Despite the fall in NLO production of E2, serum E2 started to increase on
Day 14 with a progressive rise continuing until term. A marked increase in luteal content of E2
occurred on Day 16 and continued until Day 22, whereas in vitro production of E2 by CL declined
from Day 14 onward. Serum T also began to increase on Day 14 with the peak attained between Days 18 and 20,
paralleling the rising E2 levels. In vitro production of luteal T increased on Day 12 with peak values
present between Days 16 and 20, paralleling the pattern of serum T. Maximal values of serum P occurred between Days 14 and 16 and decreased after Day 18. In
vitro production of luteal P increased abruptly on Day 4, with peak values on Day 10 and a decline
by Day 16. There was a second peak in in vitro production of P on Day 22, although serum and
luteal levels of P were already low. Changes in serum levels of 20 These findings indicate that at the end of pregnancy the CL produce large amounts of P in vitro
after functional luteolysis has occurred in vivo. The high levels of P during midpregnancy may be
involved in the suppression of follicular maturation, probably by lowering basal levels of serum LH.
Furthermore, between Days 14 and 18, secretion of T and E2 represents proportionally more
luteal than follicular sources of the hormones in the pregnant rat.
-dihydroprogesterone (20-OHP), testosterone (T), and estradiol-17
(E2) in peripheral
blood, CL, and NLO, and levels of LH and FSH in serum and pituitary were measured by radioimmunoassay.-OHP and its in vitro production
rate were inversely related to changes in P throughout pregnancy.
Note:
ACKNOWLEDGMENTS
K. T. was supported as a Ford Foundation fellow
in reproductive biology. The research was supported
by a grant from NIH (HD 00596). We wish to thank
Dr. P. F. Terranova for helping with the RIAS of
gonadotropins and for useful discussions of the research in progress. We also thank Mrs. Darlene Limback for her excellent technical assistance.
This article has been cited by other articles:
|
|
 |
|
  A. M. Benoit, H. A. LaVoie, G. L. McCoy, and C. A. Blake Expression of Sperm Protein 22 (SP22) in the Rat Ovary During Different Reproductive States Experimental Biology and Medicine, July 1, 2007; 232(7): 910 - 920. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Stocco, C. Telleria, and G. Gibori The Molecular Control of Corpus Luteum Formation, Function, and Regression Endocr. Rev., February 1, 2007; 28(1): 117 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Lee and O. D. Sherwood The Effects of Blocking the Actions of Estrogen and Progesterone on the Rates of Proliferation and Apoptosis of Cervical Epithelial and Stromal Cells During the Second Half of Pregnancy in Rats Biol Reprod, October 1, 2005; 73(4): 790 - 797. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-Y. Lee, S. Zhao, P. A. Fields, and O. D. Sherwood The Extent to which Relaxin Promotes Proliferation and Inhibits Apoptosis of Cervical Epithelial and Stromal Cells Is Greatest during Late Pregnancy in Rats Endocrinology, January 1, 2005; 146(1): 511 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Thompson, V.K.M. Han, and K. Yang Spatial and Temporal Patterns of Expression of 11{beta}-Hydroxysteroid Dehydrogenase Types 1 and 2 Messenger RNA and Glucocorticoid Receptor Protein in the Murine Placenta and Uterus During Late Pregnancy Biol Reprod, December 1, 2002; 67(6): 1708 - 1718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Seeber, J. T. Smith, and B. J. Waddell Plasma Leptin-Binding Activity and Hypothalamic Leptin Receptor Expression During Pregnancy and Lactation in the Rat Biol Reprod, June 1, 2002; 66(6): 1762 - 1767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zhao, P. A. Fields, and O. D. Sherwood Evidence That Relaxin Inhibits Apoptosis in the Cervix and the Vagina during the Second Half of Pregnancy in the Rat Endocrinology, June 1, 2001; 142(6): 2221 - 2229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. J. Waddell and P. J. Burton Full Induction of Rat Myometrial 11{beta}-Hydroxysteroid Dehydrogenase Type 1 in Late Pregnancy Is Dependent on Intrauterine Occupancy Biol Reprod, April 1, 2000; 62(4): 1005 - 1009. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. L. Russell and J. S. Richards Differentiation-Dependent Prolactin Responsiveness and Stat (Signal Transducers and Activators of Transcription) Signaling in Rat Ovarian Cells Mol. Endocrinol., December 1, 1999; 13(12): 2049 - 2064. [Abstract] [Full Text]
|
|
|
|
|
|
C. O. Stocco and R. P. Deis Luteinizing Hormone Inhibits Conversion of Pregnenolone to Progesterone in Luteal Cells from Rats on Day 19 of Pregnancy Biol Reprod, March 1, 1999; 60(3): 729 - 732. [Abstract] [Full Text]
|
|
|
|
|
|
P. J. Burton and B. J. Waddell Dual Function of 11ß-Hydroxysteroid Dehydrogenase in Placenta: Modulating Placental Glucocorticoid Passage and Local Steroid Action Biol Reprod, February 1, 1999; 60(2): 234 - 240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Burger and O. D. Sherwood Relaxin Increases the Accumulation of New Epithelial and Stromal Cells in the Rat Cervix during the Second Half of Pregnancy Endocrinology, September 1, 1998; 139(9): 3984 - 3995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nokelainen, H. Peltoketo, R. Vihko, and P. Vihko Expression Cloning of a Novel Estrogenic Mouse 17{beta}-Hydroxysteroid Dehydrogenase/ 17-Ketosteroid Reductase (m17HSD7), Previously Described as a Prolactin Receptor-Associated Protein (PRAP) in Rat Mol. Endocrinol., July 1, 1998; 12(7): 1048 - 1059. [Abstract] [Full Text]
|
|
|
|
|
|
B. J. Waddell, R. Benediktsson, R. W. Brown, and J. R. Seckl Tissue-Specific Messenger Ribonucleic Acid Expression of 11{beta}-Hydroxysteroid Dehydrogenase Types 1 and 2 and the Glucocorticoid Receptor within Rat Placenta Suggests Exquisite Local Control of Glucocorticoid Action Endocrinology, April 1, 1998; 139(4): 1517 - 1523. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Burton, Z. S. Krozowski, and B. J. Waddell Immunolocalization of 11{beta}-Hydroxysteroid Dehydrogenase Types 1 and 2 in Rat Uterus: Variation Across the Estrous Cycle and Regulation by Estrogen and Progesterone Endocrinology, January 1, 1998; 139(1): 376 - 382. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
