Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 25, 719-724, Copyright © 1981 by Society for the Study of Reproduction

Quantitative Autoradiographic Analysis of in situ Estrogen Concentration by Individual Cell Types of Mouse Target Organs: Dose-Uptake Study

C. HOLDEREGGER 1, D. A. KEEFER 1, W. BABLER 1, , and J. LANGMAN 1

1 Department of Anatomy, University of Virginia School of Medicine, Charlottesville, Virginia 22908


The pituitary, uterus, and vagina are organs which contain a diversity of cell types, many of which concentrate estrogens. In these target cells, estrogen binds to cytoplasmic receptors, migrates to the nuclei, and becomes associated with the chromatin, thereby evoking the cell's response. This study employs a quantitative autoradiographic approach to assess saturation characteristics of nuclear estrogen uptake by individual target cell types. Ovariectomized CD-1 mice were injected with 0.1, 0.3, 0.9, 2.7, or 8.1 µg/100 g BW [3H]estradiol. Sixty minutes after injection, tissues were removed and processed for quantitative dry autoradiography. In the pituitary, nuclear silver grain numbers for all five target cell types, except thyrotropes, increased with dose from 0.1 to 0.9 µg and remained unchanged from 0.9 to 8.1 µg/1OO g BW. Thyrotropes plateaued at 0.3 µg. In the uterus and vagina, nuclear silver grain numbers for six cell types increased with dose from 0.1 to 2.7 µg where they plateaued. The uptake capacity was determined for each cell type at and above saturation. These data reveal both tissue and cell type differences in saturation level and uptake capacity of [3H]estrogen in the mouse.

Note:
ACKNOWLEDGMENTS Supported by PHS grants NS06188 and HD12173 and Research Career Development Award HD00243 (D.A.K.). We wish to acknowledge the technical assistance of Ms. Bonnie Parker. We also wish to thank Dr. A. F. Parlow and NIAMDD for antisera against rLHbeta, rPRL, and rTSHbeta; and Dr. Peter Petrusz, University of North Carolina, for antisera against bGH and hbeta endorphin.

Submitted on April 23, 1981
Accepted on June 15, 1981




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Copyright © 1981 by the Society for the Study of Reproduction.