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Biology of Reproduction, Vol 25, 759-776, Copyright © 1981 by Society for the Study of Reproduction
1 Dairy Department, Institute of Food and Agricultural Sciences, College of Medicine, University of Florida, Gainesville, Florida 32611 Uterine, luteal, and follicular responses associated with the estrous cycle and early pregnancy in
cattle were examined. Dairy (n = 19) and beef (n = 19) cattle were slaughtered either on Day 4, 8,
12, 14, 16, or 19 postestrus (estrus = Day o). Corpus luteum (CL) weight, specific PGF2 In cyclic cattle, CL regression was not completed by Day 19. Follicle E2 secretion varied
among animals within day (x = 4.0-24.0 ng E2/follicle/3.5 h; P<0.01), but not among days. Total
PGF2 In summary, uterine luminal PGF increased with luteal phase of the estrous cycle at a time
when there appeared to be a stable population of specific PGF2
2 Animal Science Department, Institute of Food
and Agricultural Sciences, College of Medicine, University of Florida, Gainesville, Florida 32611
3 The Upjohn Company,
Kalamazoo, Michigan 49001
4 Department of Biochemistry, College of Medicine,
University of Florida,
Gainesville, Florida 32611
binding
by the luteal particulate fraction (100,000 x g pellet), in vitro estradiol (E2) production by the
two largest follicles, total recoverable uterine luminal protein (TP), total recoverable immunoreactive uterine luminal PGF (TPGF), and peripheral plasma steroids were evaluated. In a parallel
study, beef cattle (n = 22) were slaughtered either on Day 8, 12, 14, 16, or 19 of pregnancy for
measurements of TP, TPGF, and plasma steroids. Uterine luminal proteins, from cyclic and pregnant cattle (Day 19), were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis
(SDS-PAGE) for determination of protein molecular weights and protein profile characterization. binding (fmole/CL) for Days 4, 8, 12, 14, 16, and 19 was 14.29, 145.79, 177.34, 111.82,
174.51, and 199.17 (P<0.0l). TPGF (ng) varied among cycle Days 4 (14.4), 8 (13.9), 12 (19.7),
14 (47.7), 16 (17.4), and 19 (111.0 ng: P<0.01). In contrast, TPGF (ng) from pregnant cattle was
481.6 and 1187.8 ng on Day 16 (n = 5) and 19 (n = 6). TP (mg) in cyclic cattle varied (P<0.05)
among Days 4 (7.34), 8 (7.03), 12 (4.14), 14 (15.92), 16 (5.88), and 19 (11.35). Mean TP (mg) for
pregnant cattle ranged from 2.73 to 12.09. Thirty-two protein categories were identified in cyclic
cattle uterine flushings by SDS-PAGE (Mr range x 10-3 = 18.7 to 292.0). Proteins appeared with
greater frequency later in the cycle than earlier (Days 14, 16, 19: 60% vs Days 4, 8, 12: 45%).
Composite SDS-PAGE profiles from Days 8-12 and Days 14-16 differed (P<0.10) suggesting
luteal phase stimulation of protein secretion. Protein profiles (SDS-PAGE) from Day 19 of
pregnancy differed from Day 19 of the estrous cycle, resembled those of midluteal phase, and
revealed four protein constituents possibly unique to early pregnancy. binding sites in the CL. Higher
PGF in utero at Days 16 and 19 of pregnancy and changes in TP and SDS-PAGE protein profiles
may reflect responsiveness of endometrium to changes in ovarian status (cyclic) and/or conceptus
activity (pregnancy comparisons).
Note:
ACKNOWLEDGMENTS
This study was supported in part by U.S. Department of Agriculture, Cooperative State Research
Service Agreement No. 616-15-162.
This article has been cited by other articles:
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  M.L. Keller, A.J. Roberts, and G.E. Seidel Jr. Characterization of Insulin-Like Growth Factor-Binding Proteins in the Uterus and Conceptus during Early Conceptus Elongation in Cattle Biol Reprod, July 1, 1998; 59(3): 632 - 642. [Abstract] [Full Text]
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