Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 25, 905-914, Copyright © 1981 by Society for the Study of Reproduction

Are Lysosomes Involved in the Regression of the Male Fetal Müllerian Ducts?

DIEN TRAN 1, and NATHALIE JOSSO 1

1 Unite de Recherches de Génétique Médicale (INSERM), Hôpital des Enfants Malades, 75730 Paris Cedex 15, France


Previous studies have suggested that lysosomes play an important role in testis-mediated Müllerian regression. We have tested this hypothesis experimentally by investigating the effect of chloroquine, a lysosomotropic agent, or diazo-oxo-norleucine (DON), an inhibitor of glycosylation of lysosomal enzymes and membrane proteins, on the regression of male Müllerian ducts in organ culture. Male rat reproductive tracts together with the gonads were explanted at 14.5 days postcoitum and maintained in organ culture in control medium or in the presence of chloroquine 0.10 mM or DON 0.12 mM. Regression of Müllerian ducts was similar in all groups, although evidence for lysosomal inhibition was provided by ultrastructural and cytochemical investigations in all chloroquine-treated explants and two-thirds of the DON-treated ones. These data indicate that lysosomes are not directly involved in the effect of anti-Müllerian hormone upon its target organ.

Note:
ACKNOWLEDGMENTS The technical assistance of Miss Axelle Simon-Lorière and Miss Laurence Legeai is gratefully acknowledged. We are grateful to Dr. Jacques-Edmond Fléchon for his guidance and helpful comments and to Dr. Robert Pratt for his generous gift of diazo-oxo-norleucine.

Submitted on June 23, 1981
Accepted on July 22, 1981






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Copyright © 1981 by the Society for the Study of Reproduction.