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Biology of Reproduction, Vol 25, 945-949, Copyright © 1981 by Society for the Study of Reproduction

A Cautionary Note on the Determination of Forward Motility Protein Activity with Bovine Epididymal Spermatozoa

DONALD T. STEPUENS 1, TED S. ACOTT 1, , and DALE D. HOSKINS 1

1 Oregon Regional Primate Research Center, Beaverton, Oregon 97006


The effects of two compounds, Prosil 28 and poly G-L (a copolymer of D-glutamic acid and D-lysine), on apparent forward motility induction in bovine caput epididymal spermatozoa were examined. In a photographic forward motility assay, Prosil 28 and poly G-L both increased the apparent forward motility of spermatozoa to 50-70% of the level achievable with bovine seminal plasma, a source of forward motility protein (FMP). An analysis of the time course and sperm velocity indicated the effects were due to the ability of the agents to prevent sperm from sticking to the glass surfaces of the assay chamber, as opposed to true motility induction. Since our previous data based on this assay failed to allow for this contribution to apparent forward motility, we have concluded that our previous measurements were at least 50% too high.

Note:
ACKNOWLEDGMENTS The work described in this article, Publication No. 1168 of the Oregon Regional Primate Research Center, was supported by NIH grants RR-00163, HD-05969, and HD-11982. The authors thank Lisa Critchlow for her skillful technical assistance and Jean McKinnon for her assistance in the preparation of the manuscript.

Submitted on April 27, 1981
Accepted on August 17, 1981




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B. Nixon, R. C. Jones, and M. K. Holland
Molecular and Functional Characterization of the Rabbit Epididymal Secretory Protein 52, REP52
Biol Reprod, May 1, 2008; 78(5): 910 - 920.
[Abstract] [Full Text] [PDF]


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Copyright © 1981 by the Society for the Study of Reproduction.