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Biology of Reproduction, Vol 25, 950-957, Copyright © 1981 by Society for the Study of Reproduction
1 Department of Animal Sciences,
The University of New Hampshire,
Durham, New Hampshire 03824 To study the ability of serum to affect progesterone synthesis and its subsequent secretion into
the incubation medium, slices of bovine luteal tissue were incubated in the presence or absence of
luteinizing hormone (LH) with various types and components of sera. When serum-free incubation
medium was used, The increased secretion caused by the serum could be partially duplicated by bovine serum
albumin (BSA). Addition of BSA to the incubation medium at levels equivalent to that found in
10% bovine serum increased the progesterone secreted from 30% to 42%, but could not duplicate
the 50% secretion level achieved by the addition of the 10% serum preparations. These findings indicate that the ability of bovine luteal tissue to secrete progesteone is increased
in the presence of small amounts of serum, that this increased secretion is independent of progesterone synthesis, and that the increased secretion is not attributable to the lipoproteins in the
serum. The data support the hypothesis that serum steroid-binding proteins facilitate the transport
of steroid hormones from their site of synthesis into the blood stream.
30% of the progesterone produced was found in the incubation medium.
Addition of LH to this serum-free medium greatly increased progesterone production, but did not
increase the fraction of progesterone secreted into the incubation medium. Addition of 10% calf
serum, heat-inactivated calf serum, dialyzed calf serum, fetal calf serum, or charcoal-treated serum
to the medium increased the fraction of progesterone secreted into the incubation medium to
50%. Addition of LH to the serum-containing medium resulted in a highly significant increase in
progesterone production, but the portion of the progesterone found in the incubation medium was
no different from that found with serum alone. Removal of the lipoproteins (low and high density)
from the serum did not alter the ability of the serum to increase the secretion.
Note:
ACKNOWLEDGMENTS
This report is Scientific Contribution Number
1085 from the New Hampshire Agricultural Experiment Station. The authors are grateful to Valerie
Morrison for her excellent technical assistance. We
also wish to thank Dr. G. D. Niswender for the gift
of the progesterone antiserum, and Dr. A. E. Wilhelmi of the Hormone Distribution Program of the
NIAMDD, NIH, for the LH used in this study. This
research was supported by the Northeastern Cooperative Regional Project NE-72.
This article has been cited by other articles:
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  A. R. Liptak, B. T. Sullivan, L. E. Henkes, M. P.B. Wijayagunawardane, A. Miyamoto, J. S. Davis, B. R. Rueda, and D. H. Townson Cooperative Expression of Monocyte Chemoattractant Protein 1 Within the Bovine Corpus Luteum: Evidence of Immune Cell-Endothelial Cell Interactions in a Coculture System Biol Reprod, May 1, 2005; 72(5): 1169 - 1176. [Abstract] [Full Text] [PDF]
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