Biology of Reproduction, Vol 26, 353-366, Copyright © 1982 by Society for the Study of Reproduction

ARTICLES

Abnormal development of blastocysts and blastomeres in the rhesus monkey

AC Enders, AG Hendrickx and PE Binkerd

Preimplantation stages were collected from normal rhesus monkeys by flushing of the uterine lumen. There was a high incidence of abnormal morulae and blastocysts (25%), as well as abnormal cells and cell death in otherwise normal blastocysts. In addition to cell and nuclear fragmentation, clustering of organelles in the center of the blastomeres was a common feature in early degeneration of ova and blastomeres. Large isolated cells that lagged in cytological development were found both in large numbers in an abnormal blastocyst and as individual cells in normal blastocysts. One apparently abnormal blastocyst was composed of normal trophoblast and inner cell mass cells, but lacked desmosomes and was collapsed. The cytological study of development of the blastocyst indicated that assessment of viability of primate blastocysts by light microscopic observation of morulae and blastocyst stages is probably more fraught with difficulty than might appear from studies of common laboratory animals.

This article has been cited by other articles:

				M. Alikani, T. Schimmel, and S. M. Willadsen Cytoplasmic fragmentation in activated eggs occurs in the cytokinetic phase of the cell cycle, in lieu of normal cytokinesis, and in response to cytoskeletal disorder Mol. Hum. Reprod., May 1, 2005; 11(5): 335 - 344. [Abstract] [Full Text] [PDF]

				J. O. Gjorret, H. M. Knijn, S. J. Dieleman, B. Avery, L.-I. Larsson, and P. Maddox-Hyttel Chronology of Apoptosis in Bovine Embryos Produced In Vivo and In Vitro Biol Reprod, October 1, 2003; 69(4): 1193 - 1200. [Abstract] [Full Text] [PDF]

				A. E. Crosier, P. W. Farin, M. J. Dykstra, J. E. Alexander, and C. E. Farin Ultrastructural Morphometry of Bovine Blastocysts Produced In Vivo or In Vitro Biol Reprod, May 1, 2001; 64(5): 1375 - 1385. [Abstract] [Full Text]

				A. E. Crosier, P. W. Farin, M. J. Dykstra, J. E. Alexander, and C. E. Farin Ultrastructural Morphometry of Bovine Compact Morulae Produced In Vivo or In Vitro Biol Reprod, May 1, 2000; 62(5): 1459 - 1465. [Abstract] [Full Text]