Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hubbard, C. J.
Right arrow Articles by Terranova, P. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hubbard, C. J.
Right arrow Articles by Terranova, P. F.
Agricola
Right arrow Articles by Hubbard, C. J.
Right arrow Articles by Terranova, P. F.

Biology of Reproduction, Vol 26, 628-632, Copyright © 1982 by Society for the Study of Reproduction

ARTICLES

Inhibitory action of cyclic guanosine 5'-phosphoric acid (GMP) on oocyte maturation: dependence on an intact cumulus

CJ Hubbard and PF Terranova

Oocyte cumulus complexes (OCC) were isolated from antral follicles of proestrous hamsters prior to the luteinizing hormone (LH)/follicle- stimulating hormone (FSH) surge and then incubated from 4 to 24 h at 37 degrees C. Under these conditions approximately 89% of the oocytes exhibited metaphase plates (11% were in the dictyate stage) and addition of LH (200 ng/ml ovine NIH-LH-S20) did not significantly alter the percentage of oocyte maturation. Approximately 50% of the OCC incubated for 24 h with 6 mM 8-bromo-cyclic quanine 3':5' monophosphate (8-Br-cGMP) were prevented from maturing beyond the dictyate stage. OCC incubated with 6 mM 8-bromo-cyclic adenosine 3':5' monophosphate (8-Br- cAMP) were also prevented from maturing (59.5% 8-Br-cAMP) in vitro. LH (200 ng/ml) was able to overcome the 8-Br-cAMP-induced inhibition of oocyte maturation in OCC. It also produced a decrease in 8-Br-cGMP mediated inhibition which was more pronounced when the dosage of LH was increased to 10 microgram/ml. 8-Br-cGMP prevented oocyte maturation in a dose and time dependent manner. Only 8-Br-cAMP prevented maturation (approximately 60% inhibition) of denuded oocytes in vitro. Denuded oocytes incubated with and without 8-Br-cGMP exhibited no inhibited of maturation. These results indicate that 8-Br-cGMP may exert its inhibitory effects through the cumulus cells. On the other hand, cAMP appears to directly inhibit oocyte maturation.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
Y. Nakamura, Y. Yamagata, N. Sugino, H. Takayama, and H. Kato
Nitric Oxide Inhibits Oocyte Meiotic Maturation
Biol Reprod, November 1, 2002; 67(5): 1588 - 1592.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
I. Faerge, B. Terry, J. Kalous, P. Wahl, M. Lessl, J.L. Ottesen, P. Hyttel, and C. Grondahl
Resumption of Meiosis Induced by Meiosis-Activating Sterol Has a Different Signal Transduction Pathway than Spontaneous Resumption of Meiosis in Denuded Mouse Oocytes Cultured In Vitro
Biol Reprod, December 1, 2001; 65(6): 1751 - 1758.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Gutkowska, M. Jankowski, M. Ram Sairam, N. Fujio, A. M. Reis, S. Mukaddam-Daher, and J. Tremblay
Hormonal Regulation of Natriuretic Peptide System during Induced Ovarian Follicular Development in the Rat
Biol Reprod, July 1, 1999; 61(1): 162 - 170.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the Society for the Study of Reproduction.