Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 26, 935-942, Copyright © 1982 by Society for the Study of Reproduction


ARTICLES

Equine luteinizing hormone possesses follicle-stimulating hormone activity in hypophysectomized female rats

NR Moudgal and H Papkoff

The ability of equine luteinizing hormone (eLH) to promote follicular growth and maturation in hypophysectomized rats has been assessed. A single injection of equine LH has been shown to promote the growth of a large number of antral and preovulatory follicles. In addition, equine LH markedly increased serum estrogen levels and uterine weight. Furthermore, equine LH, like equine chorionic gonadotropin (eCG; PMSG) was able to significantly enhance the incorporation of [3H]thymidine into ovarian DNA, an activity shown to be specific to hormones having follicle-stimulating hormone (FSH) activity. Equine LH treated with an FSH antibody immunoaffinity column to remove any possible contamination still exhibited the above activity, demonstrating that the FSH activity is intrinsic to the eLH molecule. Equine LH has also been shown to be capable of inducing LH receptors in granulosa cells of ovaries of hypophysectomized rats, an activity specific to FSH-like hormones. From the doses required of eLH and the degree of response observed, it is concluded, however, that eLH in the hypophysectomized rat is less active than eCG as an FSH.


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K. Yamada, T. Mizutani, Z. Shou, T. Yazawa, T. Sekiguchi, M. Yoshino, T. Inazu, and K. Miyamoto
Cloning and Functional Expression of an E Box-Binding Protein from Rat Granulosa Cells
Biol Reprod, May 1, 2001; 64(5): 1315 - 1319.
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Copyright © 1982 by the Society for the Study of Reproduction.