Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hamilton, M. S.
Right arrow Articles by Anderson, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hamilton, M. S.
Right arrow Articles by Anderson, D. J.
Agricola
Right arrow Articles by Hamilton, M. S.
Right arrow Articles by Anderson, D. J.

Biology of Reproduction, Vol 27, 104-109, Copyright © 1982 by Society for the Study of Reproduction

ARTICLES

Antibodies to antigens on teratocarcinoma cells are associated with parity in mice

MS Hamilton and DJ Anderson

Previous studies have demonstrated that the maternal host responds immunologically to antigens of the fetus, including oncofetal antigens (OFA). However, until now, the OFA that have been studied are those associated with the postimplantation embryo. In this paper we provide evidence that the maternal host also responds immunologically to antigens associated with the mouse teratocarcinoma (TC) cell lines F9 and PYS-2, which have been established as models for the preimplantation embryo. In a solid-phase radioimmunoassay using cell membrane extracts as antigen and 125I-protein A to detect binding antibodies, sera from parous C57BL/6 mice had a significantly greater reactivity than sera from age-matched virgin animals to F9 but not PYS- 2, splenocyte or fibroblast antigen extracts. This finding was confirmed by surface immunofluorescence (IF) with fluorescein-labeled anti-mouse IgG. IF also detected a small number of multiparous mice with antibodies to PSY-2. Positive serum reactivity did not correlate directly with the number of previous litters. These TC cell lines express at least 2 OFA, F9 and Endo, which are found on preimplantation embryos and sperm, and preimmunization of female mice with F9 cells has been shown to reduce fertility. The parity-induced antibodies detected in this study could react with embryos at early stages of development and contribute to the natural decrease in litter size which occurs in mice after the second litter.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1982 by the Society for the Study of Reproduction.