Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 3, 110-119, Copyright © 1970 by Society for the Study of Reproduction

Radioimmunoassay of LH and Estrogen During the Estrous Cycle of the Ewe

R. J. SCARAMUZZI 1, B. V. CALDWELL 1, , and R. M. MOOR 1

1 Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545


A radioimmunoassay for ovine LH is described in which the plasma values are lower than those reported in most other radioimmunoassay studies. Simultaneous measurements of estrogen and LH in the ovarian venous effluent of sheep were performed by radioimmunoassay. Levels of both hormones were elevated during the follicular phase. The first rise in estrogen levels was detected 1 day before the LH rise. The measurement of LH in the peripheral and ovarian venous blood collected from the same sheep at the same time (± 5 min) indicated no significant lowering of LH in the ovarian venous effluent when compared to jugular levels.

Submitted on March 23, 1970




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Copyright © 1970 by the Society for the Study of Reproduction.