Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 3, 128-133, Copyright © 1970 by Society for the Study of Reproduction

Chromatographic Studies and Scintillation Counting of Selected Tissues of Mice Injected with Labeled Testosterone

LARS-ERIK APPELGREN 1

1 Department of Pharmacology, Royal Veterinary College, S-104 05 Stockholm 50, Sweden


Testosterone-4-14C was injected intravenously to six mice and the radioactivity was measured after 5, 20, and 60 min in the following tissues: blood, liver, kidney, adrenal, testis, ductus deferens, accessory sex glands, and brain. The highest amount of radioactivity per gram tissue among the sex organs was found in ductus deferens. Chromatography of chloroform extracts from testes, accessory sex glands, ductus deferens, and brain showed that there was a rather rapid transformation of the injected substance to a variety of labeled compounds. Quantitative differences among the different, labeled compounds in the tissues studied were registered. The ability of ductus deferens to concentrate specifically radioactivity after injection of labeled testosterone is discussed.

Submitted on March 3, 1970






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Copyright © 1970 by the Society for the Study of Reproduction.