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Biology of Reproduction, Vol 32, 203-215, Copyright © 1985 by Society for the Study of Reproduction
ARTICLES |
SK Roy and GS Greenwald
An enzymatic method was developed to collect intact follicles at different stages of development from cyclic hamsters to study ovarian folliculogenesis under various circumstances. Ovaries from 6 adult hamsters on each day of the cycle (Day 1 = ovulation) were collected, corpora lutea and large preantral and antral follicles were dissected, and follicles saved. Minced ovaries were then incubated with a mixture of collagenase, DNAse and pronase at 37 degrees C for 20 min to disperse intact follicles. Histological studies with 2191 isolated follicles revealed 10 different stages of follicular development (depending on the number of granulosa cell layers surrounding the oocyte and development of the antrum). Of the total follicular population, 14% showed signs of atresia, with 50% of those having 1-3 layers of granulosa cells (Stages 1-3); a second peak of 18% was observed in antral follicles (Stages 8-10). No signs of thecal cells were evident until the follicles reached Stage 6 (7-8 layers of granulosa cells), which possibly accounts for reduced atresia in this class and beyond. Ultrastructural study revealed that there were no signs of morphological damage to the basement membrane or to other subcellular organelles in the small preantral follicles. The presence of subnuclear lipid droplets in follicles with 3 layers of granulosa cells provided evidence for potential steroidogenesis by small follicles. The number of Stage 1-10 follicles was remarkably constant throughout the estrous cycle (460 +/- 34 per animal on Day 1 vs. 492 +/- 66 on Day 4). The usefulness of this method in analyzing follicular kinetics is illustrated in experiments involving hypophysectomy and the effects of unilateral ovariectomy. This procedure offers an improved method to study the factors responsible for the growth and the differentiation of small preantral follicles in the mammalian ovary.
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P. Yang and S. K. Roy Transforming Growth Factor B1 Stimulated DNA Synthesis in the Granulosa Cells of Preantral Follicles: Negative Interaction with Epidermal Growth Factor Biol Reprod, July 1, 2006; 75(1): 140 - 148. [Abstract] [Full Text] [PDF]
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 I Demeestere, J Centner, C Gervy, Y Englert, and A Delbaere Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents Reproduction, August 1, 2005; 130(2): 147 - 156. [Abstract] [Full Text] [PDF]
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T. Shimizu, I. Ohshima, M. Ozawa, S. Takahashi, A. Tajima, M. Shiota, H. Miyazaki, and Y. Kanai Heat stress diminishes gonadotropin receptor expression and enhances susceptibility to apoptosis of rat granulosa cells Reproduction, April 1, 2005; 129(4): 463 - 472. [Abstract] [Full Text] [PDF]
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P. Yang and S. K. Roy Follicle Stimulating Hormone-Induced DNA Synthesis in the Granulosa Cells of Hamster Preantral Follicles Involves Activation of Cyclin-Dependent Kinase-4 Rather Than Cyclin D2 Synthesis Biol Reprod, February 1, 2004; 70(2): 509 - 517. [Abstract] [Full Text] [PDF]
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S. K. Roy, J. Wang, and P. Yang Dexamethasone Inhibits Transforming Growth Factor-{beta} Receptor (T{beta}R) Messenger RNA Expression in Hamster Preantral Follicles: Possible Association with NF-YA Biol Reprod, June 1, 2003; 68(6): 2180 - 2188. [Abstract] [Full Text] [PDF]
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K. Garnett, J. Wang, and S. K. Roy Spatiotemporal Expression of Epidermal Growth Factor Receptor Messenger RNA and Protein in the Hamster Ovary: Follicle Stage-Specific Differential Modulation by Follicle-Stimulating Hormone, Luteinizing Hormone, Estradiol, and Progesterone Biol Reprod, November 1, 2002; 67(5): 1593 - 1604. [Abstract] [Full Text] [PDF]
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T. Itoh, M. Kacchi, H. Abe, Y. Sendai, and H. Hoshi Growth, Antrum Formation, and Estradiol Production of Bovine Preantral Follicles Cultured in a Serum-Free Medium Biol Reprod, October 1, 2002; 67(4): 1099 - 1105. [Abstract] [Full Text] [PDF]
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J. Mao, G. Wu, M. F. Smith, T. C. McCauley, T. C. Cantley, R. S. Prather, B. A. Didion, and B. N. Day Effects of Culture Medium, Serum Type, and Various Concentrations of Follicle-Stimulating Hormone on Porcine Preantral Follicular Development and Antrum Formation In Vitro Biol Reprod, October 1, 2002; 67(4): 1197 - 1203. [Abstract] [Full Text] [PDF]
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I. Demeestere, A. Delbaere, C. Gervy, M. Van den Bergh, F. Devreker, and Y. Englert Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice Hum. Reprod., August 1, 2002; 17(8): 2152 - 2159. [Abstract] [Full Text] [PDF]
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P. Yang, A. Kriatchko, and S. K. Roy Expression of ER-{alpha} and ER-{beta} in the Hamster Ovary: Differential Regulation by Gonadotropins and Ovarian Steroid Hormones Endocrinology, June 1, 2002; 143(6): 2385 - 2398. [Abstract] [Full Text] [PDF]
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 B. Vollmar, M. W. Laschke, R. Rohan, J. Koenig, and M. D. Menger In Vivo Imaging of Physiological Angiogenesis from Immature to Preovulatory Ovarian Follicles Am. J. Pathol., November 1, 2001; 159(5): 1661 - 1670. [Abstract] [Full Text] [PDF]
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P. Yang and S. K. Roy Epidermal Growth Factor Modulates Transforming Growth Factor Receptor Messenger RNA and Protein Levels in Hamster Preantral Follicles In Vitro Biol Reprod, September 1, 2001; 65(3): 847 - 854. [Abstract] [Full Text] [PDF]
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S. K. Roy and L. Albee Requirement for Follicle-Stimulating Hormone Action in the Formation of Primordial Follicles during Perinatal Ovarian Development in the Hamster Endocrinology, December 1, 2000; 141(12): 4449 - 4456. [Abstract] [Full Text] [PDF]
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N. Yu and S. K. Roy Development of Primordial and Prenatal Follicles from Undifferentiated Somatic Cells and Oocytes in the Hamster Prenatal Ovary In Vitro: Effect of Insulin Biol Reprod, December 1, 1999; 61(6): 1558 - 1567. [Abstract] [Full Text]
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H. Kishi and G. S. Greenwald In Vitro Steroidogenesis by Dissociated Rat Follicles, Primary to Antral, Before and After Injection of Equine Chorionic Gonadotropin Biol Reprod, November 1, 1999; 61(5): 1177 - 1183. [Abstract] [Full Text]
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