Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Herr, J. C.
Right arrow Articles by Koons, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Herr, J. C.
Right arrow Articles by Koons, D. J.
Agricola
Right arrow Articles by Herr, J. C.
Right arrow Articles by Koons, D. J.

Biology of Reproduction, Vol 32, 695-711, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Human antisperm monoclonal antibodies constructed postvasectomy

JC Herr, JE Fowler Jr, SS Howards, M Sigman, WM Sutherland and DJ Koons

Sperm and spermatogenic cell antigens, escaping the blood-testis/blood- epididymal barrier, elicit an autoimmune response in patients following vasectomy. In this study, antisperm antibody-positive sera and peripheral blood lymphocytes were obtained 6-9 mo following vasectomy. Serum antisperm antibody levels were assessed by enzyme-linked immunosorbent assay (ELISA) and indirect immunofluorescence. Lymphocyte- myeloma hybridomas were constructed by fusing peripheral blood lymphocytes, harvested from antisperm antibody-positive sera, with a hypoxanthine guanine-phosphoribosyltransferase (HGPRT)-negative mouse myeloma line. Immunoglobulin-secreting colonies surviving drug selection were detected by ELISA and screened for antisperm activity. Antisperm antibody-producing cultures were cloned and expanded for bulk antibody production both in culture and as ascites in athymic nude mice. Eight mouse-human fusions yielded 205 hybridomas secreting human monoclonal antibody, of which 11 demonstrated antisperm reactivity by ELISA. Two of these hybridomas are described in detail: HAS-1, which secretes human immunoglobulin M (IgM, kappa)-recognizing epitopes located on the sperm midpiece, and HAS-2 (IgM, lambda), which secretes monoclonal antibody-recognizing epitopes located on the entire sperm tail. The results indicate successful capture of human antisperm autoantibody from the postvasectomy autoimmune state using somatic cell hybridization techniques.


This article has been cited by other articles:


Home page
 J AndrolHome page
M. J. Wolkowicz, L. Digilio, K. Klotz, J. Shetty, C. J. Flickinger, and J. C. Herr
Equatorial Segment Protein (ESP) Is a Human Alloantigen Involved in Sperm-Egg Binding and Fusion
J Androl, May 1, 2008; 29(3): 272 - 282.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. A. Eidne, C. C. Henery, and R. J. Aitken
Selection of Peptides Targeting the Human Sperm Surface Using Random Peptide Phage Display Identify Ligands Homologous to ZP3
Biol Reprod, November 1, 2000; 63(5): 1396 - 1402.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
R. Clayton, I. D. Cooke, L. J. Partridge, and H. D.M. Moore
A Combinatorial Phage Display Library for the Generation of Specific Fab Fragments Recognizing Human Spermatozoa and Inhibiting Fertilizing Capacity In Vitro
Biol Reprod, November 1, 1998; 59(5): 1180 - 1186.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Society for the Study of Reproduction.