Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 32, 1169-1179, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Ultrastructural localization of relaxin in the corpus luteum of the nonpregnant, pseudopregnant, and pregnant pig

PA Fields and MJ Fields

Relaxin was localized in luteal cells of ovaries from nonpregnant, pseudopregnant, and pregnant pigs using porcine relaxin antiserum and peroxidase-antiperoxidase light microscopy immunohistochemistry. The number of immunoreactive cells seemed to increase from Days 17 to 106 of gestation. Luteal cells from pseudopregnant (Day 110) and nonpregnant (Day 14 of the estrous cycle) pigs were also positive for relaxin. However, less than 3% of the luteal cells in the nonpregnant animals were immunoreactive. Electron microscopy immunocytochemistry using porcine relaxin antiserum and goat antirabbit immunoglobulin G- colloidal gold demonstrated that relaxin was packaged in the small membrane-bound granules in luteal cells of pregnant as well as pseudopregnant and nonpregnant pigs. The intensity of labeling (number of gold particles) of the granules increased with pregnancy. There was a 10-fold increase in labeling of granules with the 10-nm versus 25-nm diameter gold. The goat antirabbit labeled with the smaller 10-nm gold particles was necessary to demonstrate the apparent low levels of relaxin in the luteal cells of the nonpregnant pigs. These data further indicate that pregnancy is not required for relaxin synthesis. However, physiologic significance of relaxin in corpora lutea of nonpregnant pigs has not been determined.


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U. Salli, S. Supancic, and F. Stormshak
Phosphorylation of Myristoylated Alanine-Rich C Kinase Substrate (MARCKS) Protein Is Associated with Bovine Luteal Oxytocin Exocytosis
Biol Reprod, July 1, 2000; 63(1): 12 - 20.
[Abstract] [Full Text]

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G. Min and O.D. Sherwood
Localization of Specific Relaxin-Binding Cells in the Ovary and Testis of Pigs
Biol Reprod, August 1, 1998; 59(2): 401 - 408.
[Abstract] [Full Text]


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Copyright © 1985 by the Society for the Study of Reproduction.