Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beyler, S. A.
Right arrow Articles by Goldberg, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beyler, S. A.
Right arrow Articles by Goldberg, E.
Agricola
Right arrow Articles by Beyler, S. A.
Right arrow Articles by Goldberg, E.

Biology of Reproduction, Vol 32, 1201-1210, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Binding of antibodies against antigenic domains of murine lactate dehydrogenase-C4 to human and mouse spermatozoa

SA Beyler, TE Wheat and E Goldberg

Peptide fragments of lactate dehydrogenase-C4 (LDH-C4) that contain antigenic sequences of the native protein have been identified. The present study describes the binding to murine and human spermatozoa of antibodies that were produced against synthetic peptides containing two of these sequences. Rabbits were immunized with peptides designated MC5- 15 and MC211-220, conjugated to diphtheria toxoid (DT). Antisera from these rabbits were tested for binding to washed mouse epididymal sperm or human ejaculated spermatozoa using a solid-phase radioimmunoassay. Antisera bind to mouse sperm in this system at dilutions of 1:64,000. When these antisera are first absorbed with the native LDH-C4 molecule, significant inhibition of binding to sperm results. Antisera to both DT- MC5-15 and DT-MC211-220 bind to human sperm with similar but weaker patterns than seen with mouse sperm. These data indicate that the immune response to synthetic peptides containing antigenic sequences of LDH-C4 includes antibodies that specifically bind to this enzyme on the surface of sperm. In addition, there are shared antigenic sequences between mouse and human LDH-C4, including the MC5-15 and MC211-220 peptides.


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
F. Odet, C. Duan, W. D Willis, E. H Goulding, A. Kung, E. M Eddy, and E. Goldberg
Expression of the Gene for Mouse Lactate Dehydrogenase C (Ldhc) Is Required for Male Fertility
Biol Reprod, July 1, 2008; 79(1): 26 - 34.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
J.-J. Chang, J.-P. Peng, Y. Yang, J.-L. Wang, and L. Xu
Study on the antifertility effects of the plasmid DNA vaccine expressing partial brLDH-C4'
Reproduction, January 1, 2006; 131(1): 183 - 192.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Miki, W. Qu, E. H. Goulding, W. D. Willis, D. O. Bunch, L. F. Strader, S. D. Perreault, E. M. Eddy, and D. A. O'Brien
Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility
PNAS, November 23, 2004; 101(47): 16501 - 16506.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Society for the Study of Reproduction.