Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Joshi, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Joshi, M. S.
Agricola
Right arrow Articles by Joshi, M. S.

Biology of Reproduction, Vol 33, 187-200, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Isolation and cell culture of the epithelial cells of cauda epididymidis of the bull

MS Joshi

A simple and rapid method has been described to isolate the epithelial cells of cauda epididymidis of adult bull. Perfusion of the lumen of the cauda epididymidis with 1 mg/ml collagenase in calcium- and magnesium-free Hank's balanced salt solution and incubation of the tissue at 37 degrees C for 90 min releases the principal and basal cells into the lumen. Several individual epithelial cells and cell aggregates without the contamination of stromal or smooth muscle cells can be flushed out at the end of incubation. The isolated epithelial cells, suspended in Dulbecco's medium with 10% horse serum, attach to plastic dishes within 3 h after seeding the cells and proliferate to form a monolayer in approximately 8-12 days. The electron microscopic study and immunostaining of the cultured epithelial cells indicate that the cultured cells are principal cells. The basal cells of the intact cauda epididymidis of bull show within their cytoplasm the presence of varying amounts of "lipid-like" material often closely associated with whorls of membrane.


This article has been cited by other articles:


Home page
 J AndrolHome page
Y. Araki, K. Suzuki, R. J. Matusik, M. Obinata, and M.-C. Orgebin-Crist
Immortalized Epididymal Cell Lines From Transgenic Mice Overexpressing Temperature-Sensitive Simian Virus 40 Large T-Antigen Gene
J Androl, November 1, 2002; 23(6): 854 - 869.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Society for the Study of Reproduction.