Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Salustri, A.
Right arrow Articles by Siracusa, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Salustri, A.
Right arrow Articles by Siracusa, G.
Agricola
Right arrow Articles by Salustri, A.
Right arrow Articles by Siracusa, G.

Biology of Reproduction, Vol 33, 797-802, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Effect of follicle-stimulating hormone on cyclic adenosine monophosphate level and on meiotic maturation in mouse cumulus cell- enclosed oocytes cultured in vitro

A Salustri, S Petrungaro, M De Felici, M Conti and G Siracusa

We have reported that in vitro treatment with follicle-stimulating hormone (FSH) delays by about 3 h spontaneous meiotic resumption in cumulus cell-enclosed mouse oocytes. In the present paper we show that the temporary meiotic block is accompanied by a transient increase of cAMP concentration in the oocyte. In cumulus cell-oocyte complexes stimulated with 1 microgram/ml FSH, cAMP significantly increases within 1 h both in the whole complex (from a basal value of 1.9 +/- 0.2 to 169 +/- 13 fmol) and in the enclosed oocyte (from 0.9 +/- 0.2 to 2.4 +/- 0.2 fmol), then progressively decreases to basal values. Stimulation by FSH does not cause any cAMP increase in denuded oocytes. As the concentration of cAMP in the cells decreases, the percentage of oocytes escaping the meiotic block imposed by FSH increases. If the complexes are cultured in the presence of 1 microgram/ml FSH plus 1 mM isobutyl-1- methylxanthine (1BMX), cAMP concentration increases approximately 250- fold in the complex, and 10-fold in the enclosed oocyte; the level of cAMP in the oocyte drops very rapidly (50% degradation in less than 2 min) if the oocyte is then transferred to IBMX-free medium. The data are discussed in terms of the possible role of cAMP transfer from cumulus cells to the oocyte in the regulation of meiotic progression in mouse oocytes.


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
G. Ning, H. Ouyang, S. Wang, X. Chen, B. Xu, J. Yang, H. Zhang, M. Zhang, and G. Xia
3',5'-Cyclic Adenosine Monophosphate Response Element Binding Protein Up-Regulated Cytochrome P450 Lanosterol 14{alpha}-Demethylase Expression Involved in Follicle-Stimulating Hormone-Induced Mouse Oocyte Maturation
Mol. Endocrinol., July 1, 2008; 22(7): 1682 - 1694.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Tosca, S. Uzbekova, C. Chabrolle, and J. Dupont
Possible Role of 5'AMP-Activated Protein Kinase in the Metformin-Mediated Arrest of Bovine Oocytes at the Germinal Vesicle Stage During In Vitro Maturation
Biol Reprod, September 1, 2007; 77(3): 452 - 465.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. A. Mayes, M. F. Laforest, C. Guillemette, R. B. Gilchrist, and F. J. Richard
Adenosine 5'-Monophosphate Kinase-Activated Protein Kinase (PRKA) Activators Delay Meiotic Resumption in Porcine Oocytes
Biol Reprod, April 1, 2007; 76(4): 589 - 597.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Nogueira, R. Ron-El, S. Friedler, M. Schachter, A. Raziel, R. Cortvrindt, and J. Smitz
Meiotic Arrest In Vitro by Phosphodiesterase 3-Inhibitor Enhances Maturation Capacity of Human Oocytes and Allows Subsequent Embryonic Development
Biol Reprod, January 1, 2006; 74(1): 177 - 184.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
D. Pant, L. P Reynolds, J. S Luther, P. P Borowicz, T. M Stenbak, J. J Bilski, R. M Weigl, F. Lopes, K. Petry, M. L. Johnson, et al.
Expression of connexin 43 and gap junctional intercellular communication in the cumulus-oocyte complex in sheep
Reproduction, February 1, 2005; 129(2): 191 - 200.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. J. Richard, A. Tsafriri, and M. Conti
Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation
Biol Reprod, November 1, 2001; 65(5): 1444 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K.-W. Park, S.-H. Choi, X.-X. Song, H. Funahashi, and K. Niwa
Production of Plasminogen Activators (PAs) in Bovine Cumulus-Oocyte Complexes during Maturation In Vitro: Effects of Epidermal Growth Factor on Production of PAs in Oocytes and Cumulus Cells
Biol Reprod, July 1, 1999; 61(1): 298 - 304.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. Tirone, C. D'Alessandris, V. C. Hascall, G. Siracusa, and A. Salustri
Hyaluronan Synthesis by Mouse Cumulus Cells Is Regulated by Interactions between Follicle-stimulating Hormone (or Epidermal Growth Factor) and a Soluble Oocyte Factor (or Transforming Growth Factor beta 1)
J. Biol. Chem., February 21, 1997; 272(8): 4787 - 4794.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Society for the Study of Reproduction.