Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Eppig, J. J.
Right arrow Articles by Coleman, D. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eppig, J. J.
Right arrow Articles by Coleman, D. L.
Agricola
Right arrow Articles by Eppig, J. J.
Right arrow Articles by Coleman, D. L.

Biology of Reproduction, Vol 33, 1041-1049, Copyright © 1985 by Society for the Study of Reproduction

ARTICLES

Hypoxanthine and adenosine in murine ovarian follicular fluid: concentrations and activity in maintaining oocyte meiotic arrest

JJ Eppig, PF Ward-Bailey and DL Coleman

The concentrations of hypoxanthine and adenosine in ovarian follicular fluid were estimated, using high-performance liquid chromatography, for three groups of mice: 1) pregnant mare's serum gonadotropin (PMSG)- primed mice; 2) PMSG-primed mice 2 h after injection with human chorionic gonadotropin (hCG); and 3) PMSG-primed mice 5 h after injection with hCG. The concentration of hypoxanthine in follicular fluid of Group 1 mice was 2-4 mM and of adenosine was 0.35-0.70 mM. There was no difference in the concentrations of these purines in the follicular fluid of Group 2 mice, in which maturation had been induced with hCG but the samples were taken just before germinal vesicle breakdown (GVBD). Therefore, a decrease in the concentrations of these purines does not appear to induce GVBD. A significant decrease in the concentrations of hypoxanthine and adenosine was observed in the follicular fluid of Group 3 mice in which GVBD had already occurred. This decrease was probably a result of an increase in follicular fluid volume. Adenosine had a significant, but transient, effect in maintaining both cumulus cell-enclosed and denuded oocytes in meiotic arrest; all oocytes had undergone GVBD by 100 min incubation in 1 mM adenosine. When GVBD was assessed after 3 h culture, concentrations up to 5 mM adenosine failed to maintain meiotic arrest. In contrast, hypoxanthine (2-5 mM) had a dose-dependent effect in maintaining both cumulus cell-enclosed and denuded oocytes in meiotic arrest that was sustained up to 24 h. Cumulus cell-enclosed oocytes were always more sensitive to hypoxanthine than were denuded oocytes. There was a strong synergistic effect of adenosine and hypoxanthine in maintaining meiotic arrest; 4 mM hypoxanthine and 0.75 mM adenosine maintained more than 95% of the oocytes in meiotic arrest for culture periods up to 24 h. This action was completely reversible by withdrawal of the purines. It is hypothesized that the synergistic effect of these purines may result both by promoting cyclic adenosine monophosphate synthesis (adenosine), and by preventing its hydrolysis (hypoxanthine).


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
A. DiLuigi, V. N. Weitzman, M. C. Pace, L. J. Siano, D. Maier, and L. M. Mehlmann
Meiotic Arrest in Human Oocytes Is Maintained by a Gs Signaling Pathway
Biol Reprod, April 1, 2008; 78(4): 667 - 672.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. M Schuh, B. Hille, and D. F Babcock
Adenosine and Catecholamine Agonists Speed the Flagellar Beat of Mammalian Sperm by a Non-Receptor-Mediated Mechanism
Biol Reprod, December 1, 2007; 77(6): 960 - 969.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
S. E. Edwards, M. G. Buffone, G. R. Knee, M. Rossato, G. Bonanni, S. Masiero, S. Ferasin, G. L. Gerton, S. B. Moss, and C. J. Williams
Effects of Extracellular Adenosine 5'-Triphosphate on Human Sperm Motility
Reproductive Sciences, October 1, 2007; 14(7): 655 - 666.
[Abstract] [PDF]

Home page
 Hum Reprod UpdateHome page
D. L. Russell and R. L. Robker
Molecular mechanisms of ovulation: co-ordination through the cumulus complex
Hum. Reprod. Update, May 1, 2007; 13(3): 289 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. M. Schuh, A. E. Carlson, G. S. McKnight, M. Conti, B. Hille, and D. F. Babcock
Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists
Biol Reprod, March 1, 2006; 74(3): 492 - 500.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
L. Freudzon, R. P. Norris, A. R. Hand, S. Tanaka, Y. Saeki, T. L.Z. Jones, M. M. Rasenick, C. H. Berlot, L. M. Mehlmann, and L. A. Jaffe
Regulation of meiotic prophase arrest in mouse oocytes by GPR3, a constitutive activator of the Gs G protein
J. Cell Biol., October 24, 2005; 171(2): 255 - 265.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Coticchio, G. Rossi, A. Borini, C. Grondahl, G. Macchiarelli, C. Flamigni, S. Fleming, and S. Cecconi
Mouse oocyte meiotic resumption and polar body extrusion in vitro are differentially influenced by FSH, epidermal growth factor and meiosis-activating sterol
Hum. Reprod., December 1, 2004; 19(12): 2913 - 2918.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Kawamura, J. Kumagai, S. Sudo, S.-Y. Chun, M. Pisarska, H. Morita, J. Toppari, P. Fu, J. D. Wade, R. A. D. Bathgate, et al.
Paracrine regulation of mammalian oocyte maturation and male germ cell survival
PNAS, May 11, 2004; 101(19): 7323 - 7328.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H.-Y. Fan, L.-J. Huo, D.-Y. Chen, H. Schatten, and Q.-Y. Sun
Protein Kinase C and Mitogen-Activated Protein Kinase Cascade in Mouse Cumulus Cells: Cross Talk and Effect on Meiotic Resumption of Oocyte
Biol Reprod, April 1, 2004; 70(4): 1178 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. E. Dell'Aquila, M. Albrizio, F. Maritato, P. Minoia, and K. Hinrichs
Meiotic Competence of Equine Oocytes and Pronucleus Formation after Intracytoplasmic Sperm Injection (ICSI) as Related to Granulosa Cell Apoptosis
Biol Reprod, June 1, 2003; 68(6): 2065 - 2072.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. Roberts, S. Franks, and K. Hardy
Culture environment modulates maturation and metabolism of human oocytes
Hum. Reprod., November 1, 2002; 17(11): 2950 - 2956.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. O. Arellano, A. Martinez-Torres, and E. Garay
Ionic Currents Activated via Purinergic Receptors in the Cumulus Cell-Enclosed Mouse Oocyte
Biol Reprod, September 1, 2002; 67(3): 837 - 846.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
L. M. Mehlmann, T. L. Z. Jones, and L. A. Jaffe
Meiotic Arrest in the Mouse Follicle Maintained by a Gs Protein in the Oocyte
Science, August 23, 2002; 297(5585): 1343 - 1345.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-Q. Su, K. Wigglesworth, F. L. Pendola, M. J. O'Brien, and J. J. Eppig
Mitogen-Activated Protein Kinase Activity in Cumulus Cells Is Essential for Gonadotropin-Induced Oocyte Meiotic Resumption and Cumulus Expansion in the Mouse
Endocrinology, June 1, 2002; 143(6): 2221 - 2232.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
I. Faerge, B. Terry, J. Kalous, P. Wahl, M. Lessl, J.L. Ottesen, P. Hyttel, and C. Grondahl
Resumption of Meiosis Induced by Meiosis-Activating Sterol Has a Different Signal Transduction Pathway than Spontaneous Resumption of Meiosis in Denuded Mouse Oocytes Cultured In Vitro
Biol Reprod, December 1, 2001; 65(6): 1751 - 1758.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. J. Richard, A. Tsafriri, and M. Conti
Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation
Biol Reprod, November 1, 2001; 65(5): 1444 - 1451.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Shitsukawa, C.B. Andersen, F.J. Richard, A.K. Horner, A. Wiersma, M. van Duin, and M. Conti
Cloning and Characterization of the Cyclic Guanosine Monophosphate-Inhibited Phosphodiesterase PDE3A Expressed in Mouse Oocyte
Biol Reprod, July 1, 2001; 65(1): 188 - 196.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
D. M. Taylor, A. H. Handyside, P. F. Ray, N. J. Dibb, R. M.L. Winston, and A. Ao
Quantitative measurement of transcript levels throughout human preimplantation development: analysis of hypoxanthine phosphoribosyl transferase
Mol. Hum. Reprod., February 1, 2001; 7(2): 147 - 154.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Hegele-Hartung, M. Grützner, M. Lessl, C. Grøndahl, J. L. Ottesen, and M. Brännström
Activation of Meiotic Maturation in Rat Oocytes After Treatment with Follicular Fluid Meiosis-Activating Sterol In Vitro and Ex Vivo
Biol Reprod, February 1, 2001; 64(2): 418 - 424.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
I. Faerge, C. Grøndahl, J.L. Ottesen, and P. Hyttel
Autoradiographic Localization of Specific Binding of Meiosis-Activating Sterol to Cumulus-Oocyte Complexes from Marmoset, Cow, and Mouse
Biol Reprod, February 1, 2001; 64(2): 527 - 536.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
C. Hegele-Hartung, J. Kuhnke, M. Lessl, C. Grøndahl, J. Ottesen, H. M. Beier, S. Eisner, and U. Eichenlaub-Ritter
Nuclear and Cytoplasmic Maturation of Mouse Oocytes After Treatment with Synthetic Meiosis-Activating Sterol In Vitro
Biol Reprod, November 1, 1999; 61(5): 1362 - 1372.
[Abstract] [Full Text]

Home page
 J. Lipid Res.Home page
B. Ruan, S. Watanabe, J. J. Eppig, C. Kwoh, N. Dzidic, J. Pang, W. K. Wilson, and G. J. Schroepfer , Jr.
Sterols affecting meiosis: novel chemical syntheses and the biological activity and spectral properties of the synthetic sterols
J. Lipid Res., October 1, 1998; 39(10): 2005 - 2020.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by the Society for the Study of Reproduction.