Biology of Reproduction, Vol 36, 530-535, Copyright © 1987 by Society for the Study of Reproduction

ARTICLES

Maintenance of meiotic arrest in isolated rat oocytes by the invasive adenylate cyclase of Bordetella pertussis

E Aberdam, E Hanski and N Dekel

Rat oocytes resume meiosis spontaneously in vitro within 3 h after their isolation from the ovarian follicles. We report here that the spontaneous maturation of isolated rat oocytes is preceded by a drop in intracellular levels of cyclic adenosine 3',5'-monophosphate (cAMP). Further experiments were carried out to examine the possible correlation between the meiotic status and cAMP levels within the oocyte. To challenge rat cumulus-free oocytes to generate cAMP, bypassing their own adenylate cyclase, a preparation of an invasive adenylate cyclase from Bordetella pertussis was used. We found a dose- dependent elevation of cAMP levels within these oocytes that corresponded to inhibition of their spontaneous maturation. Persistent inhibition of meiosis was obtained with the continuous presence of the enzymatic preparation, whereas its removal resulted in a transient inhibition associated with a drop in cAMP. We suggest that the presence of elevated cAMP levels in the oocyte is directly responsible for the maintenance of meiotic arrest.

This article has been cited by other articles:

				M. A. Mayes, M. F. Laforest, C. Guillemette, R. B. Gilchrist, and F. J. Richard Adenosine 5'-Monophosphate Kinase-Activated Protein Kinase (PRKA) Activators Delay Meiotic Resumption in Porcine Oocytes Biol Reprod, April 1, 2007; 76(4): 589 - 597. [Abstract] [Full Text] [PDF]

				L. B.-Y. Josefsberg, D. Galiani, S. Lazar, O. Kaufman, R. Seger, and N. Dekel Maturation-Promoting Factor Governs Mitogen-Activated Protein Kinase Activation and Interphase Suppression During Meiosis of Rat Oocytes Biol Reprod, April 1, 2003; 68(4): 1282 - 1290. [Abstract] [Full Text] [PDF]

				S. Lazar, D. Galiani, and N. Dekel cAMP-Dependent PKA Negatively Regulates Polyadenylation of c-mos mRNA in Rat Oocytes Mol. Endocrinol., February 1, 2002; 16(2): 331 - 341. [Abstract] [Full Text] [PDF]

				J.-H. Yi, L. Lefievre, C. Gagnon, M. Anctil, and F. Dube Increase of cAMP upon release from prophase arrest in surf clam oocytes J. Cell Sci., January 15, 2002; 115(2): 311 - 320. [Abstract] [Full Text] [PDF]

				F. J. Richard, A. Tsafriri, and M. Conti Role of Phosphodiesterase Type 3A in Rat Oocyte Maturation Biol Reprod, November 1, 2001; 65(5): 1444 - 1451. [Abstract] [Full Text] [PDF]

				L. B.-Y. Josefsberg, D. Galiani, A. Dantes, A. Amsterdam, and N. Dekel The Proteasome Is Involved in the First Metaphase-to-Anaphase Transition of Meiosis in Rat Oocytes Biol Reprod, May 1, 2000; 62(5): 1270 - 1277. [Abstract] [Full Text]

				C. Grøndahl, M. Lessl, I. Færge, C. Hegele-Hartung, K. Wassermann, and J. L. Ottesen Meiosis-Activating Sterol-Mediated Resumption of Meiosis in Mouse Oocytes In Vitro Is Influenced by Protein Synthesis Inhibition and Cholera Toxin Biol Reprod, March 1, 2000; 62(3): 775 - 780. [Abstract] [Full Text]