Biology of Reproduction, Vol 40, 1137-1143, Copyright © 1989 by Society for the Study of Reproduction

ARTICLES

Ontogeny of the circadian rhythm of cortisol in sheep

VH Parraguez, M Vergara, R Riquelme, R Raimann, AJ Llanos and M Seron-Ferre
Departamento de Ciencias Fisiologicas, Facultad de Ciencias Biologicas, Pontificia Universidad Catolica de Chile, Santiago.

In this work we investigated the ontogeny of the rhythm of plasma cortisol in sheep. Plasma cortisol was measured by radioimmunoassay in blood samples obtained every 1 or 2 h, for periods of 24 or 48 h, in 13 fetal sheep (124-140 days of gestation; 130.6 +/- 1.5, mean +/- SE) and in 23 newborn (5-39 days of age). To this end, indwelling polyvinyl catheters were implanted into the femoral artery and vein in all animals. The presence of rhythm was determined by Cosinor Analysis. Newborns were separated into four groups. Group 1, newborns younger than 15 days of age (7.9 +/- 0.7 days), and Group 2, newborns older than 15 days of age (25.4 +/- 2.3), were raised under nyctohemeral conditions (12L:12D). Group 3, newborns younger than 15 days of age (11.4 +/- 0.9 days), and Group 4, newborns older than 15 days of age (22.0 +/- 1.2 days), were raised under constant light conditions. A 24- h rhythm of plasma cortisol (F) was observed in newborns over 15 days of age under both nyctohemeral: F (ng/ml) = 16.1 + 7.6 cos [15 (t- 12.9)], (p = 0.01, n = 8) and constant light conditions: F (ng/ml) = 17.1 + 3.9 cos [15 (t-7.9)], (p = 0.02, n = 5). No rhythm was observed in fetal sheep or in newborn sheep younger than 15 days of age under nyctohemeral or constant light conditions.