Biology of Reproduction, Vol 43, 15-24, Copyright © 1990 by Society for the Study of Reproduction

ARTICLES

Sertoli cells, proximal convoluted tubules in the kidney, and neurons in the brain contain cyclic protein-2 [published erratum appears in Biol Reprod 1990 Sep;43(3):following 542]

SD Zabludoff, M Erickson-Lawrence and WW Wright
Department of Population Dynamics, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, Maryland 21205.

We analyze by immunocytochemistry the in vivo distribution in rat Sertoli cells of Cyclic Protein-2 (CP-2), which is maximally synthesized and secreted in vitro at stages VI and VII of the cycle of the seminiferous epithelium. This analysis demonstrates that CP-2 staining is strongest in Sertoli cells in stage VI and VII tubules. Additionally, we demonstrate that the staining for CP-2 within a stage VII tubule differs from the staining of another Sertoli cell secretory product, androgen-binding protein. CP-2 is not detected by immunocytochemistry in any other tissues of the reproductive tract, though immunoblot analysis demonstrates the presence of CP-2 in rete testis and epididymal fluids. CP-2 was immunocytochemically detected in only three other organs: the kidney, the brain (with greatest concentration in the supraoptic and paraventricular nuclei), and the posterior pituitary. The presence of CP-2 in the kidney was confirmed by metabolic radiolabeling, immunoprecipitation, and peptide analysis. The presence of CP-2 in the brain was confirmed by immunoblot analysis of radioinert protein immunoprecipitated from the anterior hypothalamus.

This article has been cited by other articles:

				M. D. Anway, W. W. Wright, B. R. Zirkin, N. Korah, J. S. Mort, and L. Hermo Expression and Localization of Cathepsin K In Adult Rat Sertoli Cells Biol Reprod, March 1, 2004; 70(3): 562 - 569. [Abstract] [Full Text] [PDF]

				W. W. Wright, L. Smith, C. Kerr, and M. Charron Mice That Express Enzymatically Inactive Cathepsin L Exhibit Abnormal Spermatogenesis Biol Reprod, February 1, 2003; 68(2): 680 - 687. [Abstract] [Full Text] [PDF]

				Y. Li, P. J. Friel, M. O. Robinson, D. J. McLean, and M. D. Griswold Identification and Characterization of Testis- and Epididymis-Specific Genes: Cystatin SC and Cystatin TE-1 Biol Reprod, December 1, 2002; 67(6): 1872 - 1880. [Abstract] [Full Text] [PDF]

				P. Sluka, L. O'Donnell, and P. G. Stanton Stage-Specific Expression of Genes Associated with Rat Spermatogenesis: Characterization by Laser-Capture Microdissection and Real-Time Polymerase Chain Reaction Biol Reprod, September 1, 2002; 67(3): 820 - 828. [Abstract] [Full Text] [PDF]

				C. C. Luedtke, S. Andonian, S. Igdoura, and L. Hermo Cathepsin A Is Expressed in a Cell- and Region-specific Manner in the Testis and Epididymis and Is Not Regulated by Testicular or Pituitary Factors J. Histochem. Cytochem., August 1, 2000; 48(8): 1131 - 1146. [Abstract] [Full Text]

				G. R. Aravindan, D. Mruk, W. M. Lee, and C. Y. Cheng Identification, Isolation, and Characterization of a 41-Kilodalton Protein from Rat Germ Cell-Conditioned Medium Exhibiting Concentration-Dependent Dual Biological Activities Endocrinology, August 1, 1997; 138(8): 3259 - 3268. [Abstract] [Full Text] [PDF]